An in vivo platform for tumor biomarker assessment Journal Article


Authors: Servais, E. L.; Suzuki, K.; Colovos, C.; Rodriguez, L.; Sima, C.; Fleisher, M.; Rusch, V. W.; Sadelain, M.; Adusumilli, P. S.
Article Title: An in vivo platform for tumor biomarker assessment
Abstract: Tumor biomarkers provide a quantitative tool for following tumor progression and response to therapy. However, investigations of clinically useful tumor biomarkers are time-consuming, costly, and limited by patient and tumor heterogeneity. In addition, assessment of biomarkers as indicators of therapy response is confounded by the concomitant use of multiple therapeutic interventions. Herein we report our use of a clinically relevant orthotopic animal model of malignant pleural mesothelioma for investigating tumor biomarkers. Utilizing multi-modality imaging with correlative histopathology, we demonstrate the utility and accuracy of the mouse model in investigating tumor biomarkers - serum soluble mesothelin-related peptide (SMRP) and osteopontin (OPN). This model revealed percentage change in SMRP level to be an accurate biomarker of tumor progression and therapeutic response - a finding consistent with recent clinical studies. This in vivo platform demonstrates the advantages of a validated mouse model for the timely and cost-effective acceleration of human biomarker translational research. © 2011 Servais et al.
Keywords: controlled study; treatment response; unclassified drug; human cell; histopathology; cisplatin; cancer growth; nonhuman; accuracy; protein blood level; animal cell; mouse; animal experiment; animal model; in vivo study; peptide; cancer model; animalia; imaging system; malignant mesothelioma; pleura mesothelioma; validity; osteopontin; soluble mesothelin related peptide; multi modality imaging; osteopontin blood level; soluble mesothelin related peptide blood level
Journal Title: PLoS ONE
Volume: 6
Issue: 10
ISSN: 1932-6203
Publisher: Public Library of Science  
Date Published: 2011-01-01
Start Page: e26722
Language: English
DOI: 10.1371/journal.pone.0026722
PROVIDER: scopus
PMCID: PMC3202552
PUBMED: 22046338
DOI/URL:
Notes: --- - "Export Date: 9 December 2011" - "Source: Scopus"
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MSK Authors
  1. Camelia S Sima
    204 Sima
  2. Valerie W Rusch
    655 Rusch
  3. Elliot Louis Servais
    17 Servais
  4. Kei Suzuki
    28 Suzuki
  5. Michel W J Sadelain
    456 Sadelain
  6. Christos Colovos
    16 Colovos
  7. Martin Fleisher
    241 Fleisher
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