Evaluation of luminex xTAG fungal analyte-specific reagents for rapid identification of clinically relevant fungi Journal Article
| Authors: | Babady, N. E.; Miranda, E.; Gilhuley, K. A. |
| Article Title: | Evaluation of luminex xTAG fungal analyte-specific reagents for rapid identification of clinically relevant fungi |
| Abstract: | Invasive fungal infections (IFI) remain a serious threat to immunocompromised hosts. Current diagnostic methods, including fungal culture and antigen detection, are slow and often lack specificity. Rapid diagnostic tools with increased sensitivity and specificity could improve the care of patients with IFI. Recently, Luminex Molecular Diagnostics (Toronto, Canada) developed 23 analyte-specific reagents (ASRs) for the detection of the most common clinically relevant fungi. This study's objective was to evaluate the sensitivity and specificity of a subset of these ASRs for fungal isolates and clinical specimens. Previously characterized fungal and bacterial isolates (n = 110), blood culture specimens (n = 34), and respiratory specimens (n = 44) were tested using either a Candida 7-plex panel (Candida albicans, Candida glabrata, Candida tropicalis, Candida parapsilosis, Candida lusitaniae, Candida guilliermondii, and Candida krusei) or a mold 11-plex panel (Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, Aspergillus terreus, Scedosporium prolificans, Scedosporium apiospermum, Fusarium oxysporum/Fusarium solani, Rhizopus arrhizus, Rhizopus microsporus, Mucor indicus, and Cunninghamella bertholletiae). The Candida 7-plex panel correctly identified all Candida isolates as confirmed by fungal culture and biochemical tests, for a sensitivity and specificity of 100%. The mold 11-plex panel correctly identified all mold isolates tested except for A. niger. Fungal isolates of Rhizopus and Mucor species were not detected, either, although they could represent species other than those targeted by the ASRs. Further evaluation will be necessary to confirm the sensitivities of some of the mold ASRs. Implementation of these ASRs will allow same-day detection of fungal DNA in clinical specimens. Copyright © 2011, American Society for Microbiology. All Rights Reserved. |
| Keywords: | controlled study; unclassified drug; nonhuman; sensitivity and specificity; bacteria (microorganisms); diagnostic value; laboratory test; bacterium isolate; diagnostic test; biochemistry; dna determination; aspergillus fumigatus; candida albicans; fungi; reagent; diagnostic test accuracy study; fungal dna; blood culture; candida; mycoses; fungus culture; fungal detection; fungus isolation; candida glabrata; analyte specific reagent; aspergillus flavus; aspergillus niger; aspergillus terreus; candida krusei; candida parapsilosis; candida tropicalis; clavispora lusitaniae; cunninghamella bertholletiae; fungus identification; fusarium oxysporum; fusarium solani; mucor; mucor indicus; pichia guilliermondii; rhizopus; rhizopus arrhizus; rhizopus microsporus; scedosporium; scedosporium apiospermum; scedosporium prolificans; chemistry, analytic; clinical laboratory techniques; mycology; fusarium; issatchenkia orientalis; pseudallescheria boydii; rhizopus oryzae |
| Journal Title: | Journal of Clinical Microbiology |
| Volume: | 49 |
| Issue: | 11 |
| ISSN: | 0095-1137 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2011-11-01 |
| Start Page: | 3777 |
| End Page: | 3782 |
| Language: | English |
| DOI: | 10.1128/jcm.01135-11 |
| PROVIDER: | scopus |
| PMCID: | PMC3209124 |
| PUBMED: | 21880976 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 9 December 2011" - "CODEN: JCMID" - "Source: Scopus" |
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