Bacterial nonhomologous end joining ligases preferentially seal breaks with a 3′-OH monoribonucleotide Journal Article

Authors: Zhu, H.; Shuman, S.
Article Title: Bacterial nonhomologous end joining ligases preferentially seal breaks with a 3′-OH monoribonucleotide
Abstract: Many bacterial species have a nonhomologous end joining system of DNA repair driven by dedicated DNA ligases (LigD and LigC). LigD is a multifunctional enzyme composed of a ligase domain fused to two other catalytic modules: a polymerase that preferentially adds ribonucleotides to double-strand break ends and a phosphoesterase that trims 3′-oligoribonucleotide tracts until only a single 3′-ribonucleotide remains. LigD and LigC have a feeble capacity to seal 3′-OH/5′-PO4 DNA nicks. Here, we report that nick sealing by LigD and LigC enzymes is stimulated by the presence of a single ribonucleotide at the broken 3′-OH end. The ribonucleotide effect on LigD and LigC is specific for the 3′-terminal nucleotide and is either diminished or abolished when additional vicinal ribonucleotides are present. No such 3′-ribonucleotide effect is observed for bacterial LigA or Chlorella virus ligase. We found that in vitro repair of a double-strand break by Pseudomonas LigD requires the polymerase module and results in incorporation of an alkali-labile ribonucleotide at the repair junction. These results illuminate an underlying logic for the domain organization of LigD, whereby the polymerase and phosphoesterase domains can heal the broken 3′-end to generate the monoribonucleotide terminus favored by the nonhomologous end joining ligases. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc.
Keywords: controlled study; unclassified drug; genetics; nonhuman; molecular genetics; protein domain; metabolism; dna damage; dna repair; genes; phosphatase; enzymology; bacteria (microorganisms); dna strand breakage; dna; double stranded dna; molecular sequence data; kinetics; nucleotide sequence; nucleic acids; substrate specificity; dna breaks, double-stranded; double stranded dna break; base sequence; organic acids; polydeoxyribonucleotide synthase; enzyme specificity; enzymes; repair; in vitro; chlorella virus; dna ligases; ligase; ligases; liga; maintenance; ribonucleotide; pseudomonas aeruginosa; polymers; bacteriology; dna ligase d; pseudomonas; ribonucleotides; joining; bacterial species; do-mains; dna repairs; strand breaks; catalytic modules; domain organizations; multi-functional; dna ligase c; nonhomologous end joining ligase; chlorella
Journal Title: Journal of Biological Chemistry
Volume: 283
Issue: 13
ISSN: 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology  
Date Published: 2008-03-28
Start Page: 8331
End Page: 8339
Language: English
DOI: 10.1074/jbc.M705476200
PUBMED: 18203718
PROVIDER: scopus
PMCID: PMC2276377
Notes: --- - "Cited By (since 1996): 8" - "Export Date: 17 November 2011" - "CODEN: JBCHA" - "Source: Scopus"
Citation Impact
MSK Authors
  1. Stewart H Shuman
    504 Shuman
  2. Hui P Zhu
    14 Zhu