Characteristics of gut microbiota in patients with rheumatoid arthritis in Shanghai, China Journal Article


Authors: Sun, Y.; Chen, Q.; Lin, P.; Xu, R.; He, D.; Ji, W.; Bian, Y.; Shen, Y.; Li, Q.; Liu, C.; Dong, K.; Tang, Y. W.; Pei, Z.; Yang, L.; Lu, H.; Guo, X.; Xiao, L.
Article Title: Characteristics of gut microbiota in patients with rheumatoid arthritis in Shanghai, China
Abstract: Little is known regarding differences in the gut microbiomes of rheumatoid arthritis (RA) patients and healthy cohorts in China. This study aimed to identify differences in the fecal microbiomes of 66 Chinese patients with RA and 60 healthy Chinese controls. The V3-V4 variable regions of bacterial 16S rRNA genes were sequenced with the Illumina system to define the bacterial composition. The alpha-diversity index of the microbiome of the RA patients was significantly lower than that of the control group. The bacterial genera Bacteroides (p = 0.02202) and Escherichia-Shigella (p = 0.03137) were more abundant in RA patients. In contrast, Lactobacillus (p = 0.000014), Alloprevotella (p = 0.0000008615), Enterobacter (p = 0.000005759), and Odoribacter (p = 0.0000166) were less abundant in the RA group than in the control group. Spearman correlation analysis of blood physiological measures of RA showed that bacterial genera such as Dorea and Ruminococcus were positively correlated with RF-IgA and anti-CCP antibodies. Furthermore, Alloprevotella and Parabacteroides were positively correlated with the erythrocyte sedimentation rate, and Prevotella-2 and Alloprevotella were positively correlated with C-reactive protein, both biomarkers of inflammation. These findings suggest that the gut microbiota may contribute to RA development via interactions with the host immune system. © Copyright © 2019 Sun, Chen, Lin, Xu, He, Ji, Bian, Shen, Li, Liu, Dong, Tang, Pei, Yang, Lu, Guo and Xiao.
Keywords: adult; controlled study; middle aged; gene sequence; human cell; major clinical study; pathogenesis; nonhuman; polymerase chain reaction; biological marker; c reactive protein; inflammation; cohort analysis; biomarker; escherichia coli; rheumatoid arthritis; real time polymerase chain reaction; intestine flora; rna 16s; dna extraction; erythrocyte sedimentation rate; china; bacteroides; microbial diversity; actinobacteria; bacteroidetes; firmicutes; prevotella; proteobacteria; feces microflora; shigella; human; male; female; article; gut microbiome; 16s rrna gene sequencing; alloprevotella; parabacteroides; ruminococcus; species composition
Journal Title: Frontiers in Cellular and Infection Microbiology
Volume: 9
ISSN: 2235-2988
Publisher: Frontiers Media S.A.  
Date Published: 2019-10-23
Start Page: 369
Language: English
DOI: 10.3389/fcimb.2019.00369
PUBMED: 31709198
PROVIDER: scopus
PMCID: PMC6819506
DOI/URL:
Notes: Article -- Export Date: 2 December 2019 -- Source: Scopus
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  1. Yi-Wei Tang
    188 Tang