RUNX2 (6p21.1) amplification in osteosarcoma Journal Article
| Authors: | Gupta, S.; Ito, T.; Alex, D.; Vanderbilt, C. M.; Chang, J. C.; Islamdoust, N.; Zhang, Y.; Nafa, K.; Healey, J.; Ladanyi, M.; Hameed, M. R. |
| Article Title: | RUNX2 (6p21.1) amplification in osteosarcoma |
| Abstract: | Prior cytogenetic profiling of osteosarcomas has suggested that amplifications at the 6p12-21 locus are relatively common alterations in these tumors. However, these studies have been limited by variable testing methodologies used as well as by the relatively small numbers of cases that have been analyzed. To better define the frequency of this alteration, 111 osteosarcomas were profiled using hybridization capture-based next-generation sequencing (NGS) platform (Memorial Sloan Kettering Integrated Mutation Profiling of Actionable Cancer Targets) as part of an institutional clinical cancer genomics initiative. Using this platform, amplification at the 6p12-21 locus was determined by copy number assessment of the VEGFA and CCND3 genes. In addition, fluorescence in situ hybridization was used to assess copy number status for RUNX2, a known transcriptional regulator of osteoblastic differentiation which has previously been reported to be dysregulated in osteosarcomas. 6p12-21 amplification using NGS-based copy number assessment was confirmed in more than a fifth of all cases tested (24 of 111, 21.6%). Most of these cases, when tested using fluorescence in situ hybridization, were found to include RUNX2 within the amplified locus (17 of 18, 94.4%). Whereas many laboratories lack access to large-panel NGS assays, the use of fluorescence in situ hybridization to identify 6p12-21 amplification events by targeting RUNX2 represents a widely available diagnostic modality for the identification of such cases. This could help better define the role of RUNX2 in osteoblastic differentiation and serve as a surrogate for the identification of potentially targetable alterations such as VEGFA amplification at this locus. © 2019 Elsevier Inc. |
| Keywords: | osteosarcoma; amplification; runx2; 6p12; 6p21 |
| Journal Title: | Human Pathology |
| Volume: | 94 |
| ISSN: | 0046-8177 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2019-12-01 |
| Start Page: | 23 |
| End Page: | 28 |
| Language: | English |
| DOI: | 10.1016/j.humpath.2019.09.010 |
| PUBMED: | 31669178 |
| PROVIDER: | scopus |
| PMCID: | PMC7053429 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 2 December 2019 -- Source: Scopus |
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