In vivo imaging with confirmation by histopathology for increased rigor and reproducibility in translational research: A review of examples, options, and resources Journal Article


Authors: Gabrielson, K.; Maronpot, R.; Monette, S.; Mlynarczyk, C.; Ramot, Y.; Nyska, A.; Sysa-Shah, P.
Article Title: In vivo imaging with confirmation by histopathology for increased rigor and reproducibility in translational research: A review of examples, options, and resources
Abstract: Preclinical noninvasive imaging can be an indispensable tool for studying animal models of disease. In vivo imaging to assess anatomical, functional, and molecular features requires verification by a comparison to the macroscopic and microscopic morphological features, since all noninvasive in vivo imaging methods have much lower resolution than standard histopathology. Comprehensive pathological evaluation of the animal model is underutilized; yet, many institutions have veterinary or human pathologists with necessary comparative pathology expertise. By performing a rigorous comparison to gross or histopathology for image interpretation, these trained individuals can assist scientists with the development of the animal model, experimental design, and evaluation of the in vivo imaging data. These imaging and pathology corroboration studies undoubtedly increase scientific rigor and reproducibility in descriptive and hypothesis-driven research. A review of case examples including ultrasound, nuclear, optical, and MRI is provided to illustrate how a wide range of imaging modalities data can be confirmed by gross or microscopic pathology. This image confirmation and authentication will improve characterization of the model and may contribute to decreasing costs and number of animals used and to more rapid translation from preclinical animal model to the clinic. © 2018 The Author(s) 2018. Published by Oxford University Press on behalf of the National Academy of Sciences. All rights reserved.
Keywords: ct; mri; optical imaging; ultrasound imaging; gross and histopathology; pet and spect; preclinical imaging; rigor and reproducibility
Journal Title: ILAR Journal
Volume: 59
Issue: 1
ISSN: 1084-2020
Publisher: Oxford University Press  
Date Published: 2018-01-01
Start Page: 80
End Page: 98
Language: English
DOI: 10.1093/ilar/ily010
PUBMED: 30541081
PROVIDER: scopus
PMCID: PMC6645176
DOI/URL:
Notes: Review -- Source: Scopus
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  1. Sebastien Monette
    149 Monette