Amplification of JC virus DNA from brain and cerebrospinal fluid of patients with progressive multifocal leukoencephalopathy Journal Article
| Authors: | Henson, J.; Rosenblum, M.; Armstrong, D.; Furneaux, H. |
| Article Title: | Amplification of JC virus DNA from brain and cerebrospinal fluid of patients with progressive multifocal leukoencephalopathy |
| Abstract: | We have established an assay to detect JC viral DNA in brain tissue and CSF of patients with progressive multifocal leukoencephalopathy (PML) and amplified a 106-base pair segment of the gene encoding JC viral large T antigen by polymerase chain reaction (PCR) from the brains of eight patients with PML and from one of six CSF samples. Brain and C SF of patients without PML did not yield JC virus-specific DNA amplification products. Southern analysis documented that the sequences of the 106-base pair PCR product and JC viral DNA are identical. This assay can be used in the diagnosis of PML and to investigate the biology of JC virus. © 1991 American Academy of Neurology. |
| Keywords: | clinical article; controlled study; human tissue; human cell; polymerase chain reaction; gene amplification; molecular sequence data; brain; cerebrospinal fluid; virus large t antigen; base sequence; dna, viral; tumor virus infections; base pairing; dna sequence; progressive multifocal leukoencephalopathy; leukoencephalopathy, progressive multifocal; leukocyte count; virus detection; oligonucleotides; virus dna; brain tissue; blotting, southern; human; priority journal; article; jc virus; support, u.s. gov't, p.h.s. |
| Journal Title: | Neurology |
| Volume: | 41 |
| Issue: | 12 |
| ISSN: | 0028-3878 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 1991-12-01 |
| Start Page: | 1967 |
| End Page: | 1971 |
| Language: | English |
| PUBMED: | 1660572 |
| PROVIDER: | scopus |
| DOI: | 10.1212/wnl.41.12.1967 |
| DOI/URL: | |
| Notes: | Source: Scopus |
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