Development of the immunofluorescent antibody test for detection of feline leukemia virus infection in cats Conference Paper
| Authors: | Hardy, W. D. Jr; Zuckerman, E. E. |
| Title: | Development of the immunofluorescent antibody test for detection of feline leukemia virus infection in cats |
| Conference Title: | Colloquium on Feline Leukemia Virus/Feline Immunodeficiency Virus: Tests and Vaccination |
| Abstract: | Studies of the immunodetection of various microorganisms by various assay systems indicated that the most specific and sensitive assays are immunofluorescence, radioimmunoassay, and immunoblot analysis (western blot), followed by sensitive but less specific ELISA and agglutination assays and, finally, by even less sensitive but very specific virus isolation and double immunodiffusion techniques. The first test for the clinical detection of FeLV infection in pet cats was the immunofluorescent antibody (IFA) test, which was introduced in 1972. The FeLV test is used for detection for FeLV infection and not as a test for leukemia or any other feline disease. The IFA test was compared with an immunodiffusion (ID) test and with tissue culture isolation (TCI) of the virus in 26 cats to establish a standard for FeLV tests. Excellent correlation was observed between the IFA and the ID tests (100%). |
| Keywords: | comparative study; animal; animals; fluorescent antibody technique; blood; immunology; cat; cat disease; cats; prediction and forecasting; predictive value of tests; virus antigen; isolation and purification; antiserum; antigens, viral; immune sera; article; feline leukemia virus; immunodiffusion; leukemia virus, feline; leukemia, feline |
| Journal Title | Journal of the American Veterinary Medical Association |
| Volume: | 199 |
| Issue: | 10 |
| Conference Dates: | 1991 Mar 1-3 |
| Conference Location: | Orlando, FL |
| ISBN: | 0003-1488 |
| Publisher: | American Veterinary Medical Association |
| Date Published: | 1991-11-15 |
| Start Page: | 1327 |
| End Page: | 1335 |
| Language: | English |
| PUBMED: | 1666076 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
