Induction of IgG antibodies against GD3 ganglioside in rabbits by an anti-idiotypic monoclonal antibody Journal Article

   


Authors: Chapman, P. B.; Houghton, A. N.
Article Title: Induction of IgG antibodies against GD3 ganglioside in rabbits by an anti-idiotypic monoclonal antibody
Abstract: Anti-idiotypic MAb were raised in syngeneic mice against a mouse MAb recognizing GD3ganglioside (MAb R24). Two anti-idiotypic MAb, designated BEC2 and BEC3, recognized distinct determinants on MAb R24 that mapped near or within the GD3-binding site. New Zealand white rabbits, which express GD3 on normal tissues, were immunized with either BEC2, BEC3, or control MAb FLOPC-21. All rabbits developed high and equivalent titers of antibodies against mouse immunoglobulins. Immunization with BEC2 and BEC3 induced rabbit antibodies expressing R24 idiotype as demonstrated by their ability to inhibit BEC2 binding to R24. Antibodies (IgG and IgM) reacting with GD3 developed in five of eight rabbits immunized with BEC2 but not in rabbits immunized with BEC3 or with control MAb. Serum antibodies against GD3 did not cross-react with other gangliosides. These results show that MAb BEC2 can mimic GD3 ganglioside and can induce antibodies against GD3 ganglioside despite expression of GD3 on normal rabbit tissue.
Keywords: nonhuman; animal; mice; animal tissue; melanoma; animal experiment; mice, inbred balb c; mice, inbred c57bl; monoclonal antibody; antibodies, monoclonal; immunoglobulin g; immunization; glycolipid; gangliosides; rabbits; idiotypic antibody; antibodies, anti-idiotypic; rabbit; immunoglobulin idiotypes; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; internal image
Journal Title: Journal of Clinical Investigation
Volume: 88
Issue: 1
ISSN: 0021-9738
Publisher: American Society for Clinical Investigation  
Date Published: 1991-07-01
Start Page: 186
End Page: 192
Language: English
DOI: 10.1172/jci115276
PUBMED: 2056117
PROVIDER: scopus
PMCID: PMC296019
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025831938&doi=10.1172%2fJCI115276&partnerID=40&md5=56ff27cde22b3ee4cb45f3a9e52f7171
Notes: Article -- Export Date: 27 September 2019 -- Source: Scopus
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MSK Authors
  1. Paul Chapman
    326 Chapman
  2. Alan N Houghton
    364 Houghton
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