| Abstract: |
The purpose of this study was to isolate mouse monoclonal antibodies (MAbs) recognizing Galα I ‐4Galβ I −4Glc (Pk. CD77), which has been described as a Burkitt's‐lym homa‐associated using a synthetic glycoconjugate as immunogen and a filter immunoplaque screening assay. One MAb (PK67) was characterized by immuno‐thin‐layer chromatography, ELISA and competition assays using neutral glycolipids from various sources and a variety of carbohydrates and glycoproteins. Epitope analysis showed that all 3 carbohydrate moieties are required in PK67 binding. No cross‐reactivity was observed with closely related carbohydrate structures, with the exception of Galα I ‐4Ga1β I ‐4GlcNAc. However, the reactivity with this structure was several orders of magnitude lower than with Pk. lmmunohistochemical analysis of fresh‐frozen tissue specimens showed that Gb3, is widely expressed: prominent staining was observed in many normal tissues, e.g., kidney and gastric tissue and on capillary endothelial cells. In lymph nodes, very weak staining of a few B cells was observed. Flow cytometric analysis of hematopoietic neoplasms showed Gb, expression on B‐cell neoplasms, particularly those with committed 8‐cell phenotype indicating that, in addition to earlier reports of Gb3, expression on Burkitt's lymphoma, Gb3, is present on other committed B‐cell neoplasms. Copyright © 1991 Wiley‐Liss, Inc., A Wiley Company |
| Keywords: |
controlled study; human tissue; leukemia; neoplasms; animal; mice; tumor antigen; mice, inbred balb c; monoclonal antibody; immunoenzyme techniques; antibodies, monoclonal; molecular sequence data; reference values; lymphoma; organ specificity; antigens, cd; enzyme-linked immunosorbent assay; glycoproteins; burkitt lymphoma; carbohydrate sequence; carbohydrates; glycolipids; hybridomas; cross reactions; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; trihexosylceramides
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