Molecular abnormalities in the expression of HLA class‐I antigens by melanoma cells Journal Article
| Authors: | Wang, Z.; Cao, Y.; D'Urso, C.; Houghton, A. N.; Ferrone, S. |
| Article Title: | Molecular abnormalities in the expression of HLA class‐I antigens by melanoma cells |
| Abstract: | Testing of a panel of cultured human melanoma cells with radiolabelled anti‐HLA‐class‐l monoclonal antibodies (MAbs) in a binding assay has shown lack of reactivity of FO‐I and SK‐MEL‐33 cells and low reactivity of SK‐MEL‐19 cells. SDS‐PAGE analysis of the components immunoprecipitated from the 3 intrinsically radiolabelled melanoma cell lines by antibodies to the 2 subunits of HLA‐class‐I antigens has not detected β2‐μ in the immunoprecipitates from melanoma cells FO‐I and SK‐MEL‐33 and only a low level of HLA‐class‐I heavy chain in the immunoprecipitate from SK‐MEL‐19 cells. Northern blotting analysis with probes specific for HLA‐class‐I heavy chain and for β2‐μ indicates that the abnormalities in HLA‐class‐I‐antigen expression reflects a defect at the transcriptional level in FO‐1 cells and at the post‐transcriptional level in SK‐MEL‐19 and in SK‐MEL‐33 cells. FO‐1, SK‐MEL‐19 and SK‐MEL‐33 cells represent useful models to analyze the molecular mechanisms underlying the loss of HLA‐class‐I‐antigen expression which is often associated with malignant transformation of melanocytes and to characterize the role of HLA‐class‐I antigens in the biology of melanoma cells and in their interactions with effector cells. Copyright © 1991 Wiley‐Liss, Inc., A Wiley Company |
| Keywords: | controlled study; human cell; genetics; conference paper; antigen expression; gene; melanoma; gene expression; cell line; genetic transcription; transcription, genetic; monoclonal antibody; immunology; antibodies, monoclonal; messenger rna; rna, messenger; cancer cell; hla antigen class 1; histocompatibility antigens class i; antibodies; isolation and purification; blotting, northern; antibody; polyacrylamide gel electrophoresis; electrophoresis, polyacrylamide gel; northern blotting; genes, mhc class i; human; priority journal; article; support, u.s. gov't, p.h.s. |
| Journal Title: | International Journal of Cancer |
| Volume: | 47 |
| Issue: | Suppl. 6 |
| ISSN: | 0020-7136 |
| Publisher: | John Wiley & Sons |
| Date Published: | 1991-01-01 |
| Start Page: | 101 |
| End Page: | 105 |
| Language: | English |
| DOI: | 10.1002/ijc.2910470720 |
| PUBMED: | 2066175 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
