Hybridoma-derived human suppressor factors: Inhibition of growth of tumor cell lines and effect on cytotoxic cells Journal Article
| Authors: | Kunicka, J. E.; Fox, F. E.; Seki, H.; Oleszak, E. L.; Platsoucast, C. D. |
| Article Title: | Hybridoma-derived human suppressor factors: Inhibition of growth of tumor cell lines and effect on cytotoxic cells |
| Abstract: | With the objective of developing human T-T cell hybrids producing B-cell growth factor, we fused concavalin A–activated T lymphocytes with cells of the lurkat T cell line. The hybrids were selected on the basis of their ability to form colonies in soft agar, whereas the parent Jurkat T cell line did not. T-T cell hybrids were HLA-typed, screened by functional tests, and recloned by limiting dilution. In addition to obtaining B-cell growth factor-producing hybrids, we also obtained certain other T-T cell hybrids (as determined by HLA-typing) producing suppressor factors inhibiting proliferative responses and antibody production by human lymphocytes. Subsequently, a suppressor factor with similar inhibitory properties was identified in supernatants of the Jurkat T cell line. However, the Jurkat factor exhibited different biochemical and functional properties than the hybridoma-derived suppressor factors. Using two-parameter cell cycle analysis and the metachromatic fluorochrome acridine orange, we found that the hybridoma-derived 160 and 169 suppressor factors arrested phytohemagglutinin-induced proliferation of peripheral blood mononuclear cells in the G0G1 phase of the cell cycle, whereas the Jurkat suppressor factor arrested proliferation in the S phase. Incubation of peripheral blood mononuclear cells with the 160, 169, or Jurkat suppressor factors for 24 hr at 37° C, followed by washing, did not alter their cell cycle progression (or RNA content) in response to stimulation with phytohemagglutinin. The hybridoma-derived 160 and 169 suppressor factors and the Jurkat factor inhibited the growth but not the viability of cells from the following human tumor cell lines: A673 sarcoma cell line, SK-LC-6 and SK-LC-14 lung cell lines, SB, Raji, and Daudi lymphoblastoid cell lines, and FARR malignant melanoma cell line. In contrast, it did not affect the growth of murine L1210 cells and FS-4 normal human diploid fibroblasts. The hybridoma-derived 160 suppressor factor was selected to investigate its effect on cell-mediated cytotoxicity: The 160 suppressor factor did not inhibit natural killer cytotoxicity or its augmentation by interferon alpha or interleukin 2 or the generation of lymphokine-activated killer cells. However, this factor partially inhibited the generation of specific T cell-mediated cytotoxicity. © 1991 Butterworth–Heinemann. |
| Keywords: | controlled study; human cell; t lymphocyte; t-lymphocytes; cell cycle; cytotoxicity; tumor cells, cultured; lymphocyte activation; cytotoxicity, immunologic; hybridomas; human; article; support, u.s. gov't, p.h.s.; phytohemagglutinins; human t-t cell hybrids; inhibition of tumor cell growth; suppressor factors; suppressor factors, immunologic |
| Journal Title: | Human Antibodies |
| Volume: | 2 |
| Issue: | 3 |
| ISSN: | 1093-2607 |
| Publisher: | IOS Press |
| Date Published: | 1991-07-01 |
| Start Page: | 160 |
| End Page: | 169 |
| Language: | English |
| DOI: | 10.3233/hab-1991-2307 |
| PUBMED: | 1873505 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Chris D. Platsoucas' last name is misspelled on PDF -- Article -- Export Date: 27 September 2019 -- Source: Scopus |
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