Identification and expression analysis of novel LAGE-1 alleles with single nucleotide polymorphisms in cancer patients Journal Article
| Authors: | Shao, Y.; Sun, Z. Y.; Sun, S. W.; Zhao, Y.; Sin, W. Y.; Yuan, Y. H.; Simpson, A. J.; Old, L. J.; Sang, X. T.; Mao, Y. L.; Xie, Y.; Huang, J. F.; Zhao, H. T. |
| Article Title: | Identification and expression analysis of novel LAGE-1 alleles with single nucleotide polymorphisms in cancer patients |
| Abstract: | Objective: The purpose of this study is to identify single nucleotide polymorphisms (SNPs) in the coding region alleles of the X chromosomal LAGE-1 gene, and investigate the frequency of such SNPs in both cancer patients and healthy controls, and thus determine the potential significance of these SNPs with respect to cancer vaccine therapy. Methods: In this study, different mRNAs transcribed from the LAGE-1 gene were identified by RT-PCR from healthy donors and cancer patients samples. Results: A new LAGE-1 allele containing three coding region SNPs (69A/G, 317C/G, and 397T/G) were identified. The allele is highly expressed as the LAGE-1a mRNA variant AY679089 in some of the cancer patients. The three SNPs altered the LAGE-1 gene sequence to that of NYESO-1 at both the nucleotide and amino acid level. Conclusion: There is a high frequency of the LAGE-1 gene allele with SNPs in coding regions in cancer patients. There was a clear relationship between the variant AY679089 and gastric cancer. The SNPs may lead to accelerated progress of poorly differentiated gastric cancer. The SNPs found in these alleles may also alter the immunological characteristics of LAGE-1a and should be taken into account if this antigen is adopted as a cancer vaccine component. © 2007 Springer-Verlag. |
| Keywords: | controlled study; human tissue; gene sequence; human cell; major clinical study; single nucleotide polymorphism; polymorphism, single nucleotide; cancer patient; neoplasms; allele; gene; reverse transcription polymerase chain reaction; gene expression; alleles; cancer therapy; molecular sequence data; antigens, neoplasm; messenger rna; gene identification; nucleotide sequence; base sequence; amino acid; stomach neoplasms; vaccine; antigens, surface; nucleotide; lage-1 gene; transcript variant; lage 1 gene |
| Journal Title: | Journal of Cancer Research and Clinical Oncology |
| Volume: | 134 |
| Issue: | 4 |
| ISSN: | 0171-5216 |
| Publisher: | Springer |
| Date Published: | 2008-04-01 |
| Start Page: | 495 |
| End Page: | 502 |
| Language: | English |
| DOI: | 10.1007/s00432-007-0312-z |
| PUBMED: | 17899192 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Export Date: 17 November 2011" - "CODEN: JCROD" - "Molecular Sequence Numbers: GENBANK: AJ223041, AY679089;" - "Source: Scopus" |
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