Heat shock protein hsp70 protects cells from thermal stress even after deletion of its ATP-binding domain Journal Article
| Authors: | Li, G. C.; Li, L.; Liu, R. Y.; Rehman, M.; Lee, W. M. F. |
| Article Title: | Heat shock protein hsp70 protects cells from thermal stress even after deletion of its ATP-binding domain |
| Abstract: | Retroviral-mediated gene transfer experiments show that rodent cells become heat resistant when stably and constitutively expressing a cloned human gene encoding an intact human 70-kDa heat shock protein (hsp70). Cells expressing higher levels of the hsp70 protein generally tolerate thermal stress better, whereas cells expressing either of two mutated hsp70-encoding genes, one with a 4-base pair out-of-frame. deletion and one with an in-frame deletion of codons 438-618, are heat sensitive. These results provide strong evidence that expression of hsp70 leads directly to thermal tolerance. Surprisingly, cells expressing a mutant hsp70 of a human gene missing codons 120-428 are, nevertheless, heat resistant. Because the deleted region of this mutant contains the ATP-binding domain of human hsp70, this domain appears dispensable in the hsp70-mediated protection of cells from thermal stress. |
| Keywords: | gene; retrovirus; thermotolerance; stable expression; expression; induction; recovery; localization; construction; atp binding; chinese-hamster fibroblasts; resistant variants; thermal resistance |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 89 |
| Issue: | 6 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1992-03-15 |
| Start Page: | 2036 |
| End Page: | 2040 |
| Language: | English |
| ACCESSION: | WOS:A1992HJ05300009 |
| DOI: | 10.1073/pnas.89.6.2036 |
| PROVIDER: | wos |
| PMCID: | PMC48591 |
| PUBMED: | 1549562 |
| Notes: | Article -- Source: Wos |
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