Evaluation of epidermal growth factor receptor DNA amplification and mRNA expression in bladder cancer Journal Article

  

Authors:	Wood, D. P. Jr; Fair, W. R.; Chaganti, R. S. K.
Article Title:	Evaluation of epidermal growth factor receptor DNA amplification and mRNA expression in bladder cancer
Abstract:	The epidermal growth factor receptor has been implicated in the malignant transformation of cells because the v-erbB oncogene is a truncated form of the epidermal growth factor receptor. DNA amplification and/or mRNA overexpression of the epidermal growth factor receptor is associated with the malignant potential of several human epithelial tumors. To determine the frequency of epidermal growth factor receptor DNA amplification and mRNA overexpression in bladder cancer, we evaluated 12 bladder tumors for DNA amplification and another 14 bladder tumors for mRNA overexpression. By Southern hybridization, we found no evidence of DNA amplification or gene rearrangements in 12 bladder tumors. Five of 14 bladder tumors overexpressed epidermal growth factor receptor mRNA four to 15 fold compared to normal urothelium from the same bladder by Northern analysis. These findings suggest that epidermal growth factor receptor amplification and/or gene rearrangement occurs infrequently in bladder cancer. Epidermal growth factor receptor mRNA overexpression, however, is found in 36% of bladder tumors and may play a role in bladder tumorogenesis.
Keywords:	gene; epidermal growth factor receptor; kidney; tumors; factor-alpha; cell-carcinoma; neoplasms, bladder
Journal Title:	Journal of Urology
Volume:	147
Issue:	1
ISSN:	0022-5347
Publisher:	Elsevier Science, Inc.
Date Published:	1992-01-01
Start Page:	274
End Page:	277
Language:	English
ACCESSION:	WOS:A1992GX78200087
DOI:	10.1016/s0022-5347(17)37212-9
PROVIDER:	wos
PUBMED:	1729545
Notes:	Article -- Source: Wos

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