The purified yeast pre-mRNA splicing factor PRP2 is an RNA-dependent NTPase Journal Article
| Authors: | Kim, S. H.; Smith, J.; Claude, A.; Lin, R. J. |
| Article Title: | The purified yeast pre-mRNA splicing factor PRP2 is an RNA-dependent NTPase |
| Abstract: | Unlike autocatalyzed self-splicing reactions, nuclear pre-mRNA splicing requires transacting macromolecules and ATP. A protein encoded by the PRP2 gene of Saccharomyces cerevisiae is required, in conjunction with ATP, for the first cleavage-ligation reaction of pre-mRNA splicing. In this study, we have purified two forms of the PRP2 gene product with apparent molecular weights of 100 kDa and 92 kDa, from a yeast strain overproducing the protein. Both proteins were indistinguishable in their ability to complement extracts derived from a heat-sensitive prp2 mutant. Furthermore, we show that the PRP2 protein is capable of hydrolyzing nucleoside triphosphates in the presence of single-stranded RNAs such as poly(U). However, purified PRP2 by itself did not unwind double-stranded RNA substrates. The fact that an RNA-dependent NTPase activity is intrinsic to PRP2 may account for the ATP requirement in the first catalytic reaction of pre-mRNA splicing. |
| Keywords: | protein; saccharomyces-cerevisiae; identification; escherichia-coli; splicing factors; invitro; helicase activity; atpases; dead-box proteins; pre-messenger-rna splicing |
| Journal Title: | EMBO Journal |
| Volume: | 11 |
| Issue: | 6 |
| ISSN: | 0261-4189 |
| Publisher: | Wiley Blackwell |
| Date Published: | 1992-06-01 |
| Start Page: | 2319 |
| End Page: | 2326 |
| Language: | English |
| ACCESSION: | WOS:A1992HX08100035 |
| DOI: | 10.1002/j.1460-2075.1992.tb05291.x |
| PROVIDER: | wos |
| PMCID: | PMC556699 |
| PUBMED: | 1534753 |
| Notes: | Source: Wos |
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