Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst Journal Article


Authors: Plusa, B.; Piliszek, A.; Frankenberg, S.; Artus, J.; Hadjantonakis, A. K.
Article Title: Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst
Abstract: The first two lineages to differentiate from a pluripotent cell population during mammalian development are the extraembryonic trophectoderm (TE) and the primitive endoderm (PrE). Whereas the mechanisms of TE specification have been extensively studied, segregation of PrE and the pluripotent epiblast (EPI) has received comparatively little attention. A current model of PrE specification suggests PrE precursors exhibit an apparently random distribution within the inner cell mass of the early blastocyst and then segregate to their final position lining the cavity by the late blastocyst. We have identified platelet-derived growth factor receptor alpha (Pdgfrα) as an early-expressed protein that is also a marker of the later PrE lineage. By combining live imaging of embryos expressing a histone H2B-GFP fusion protein reporter under the control of Pdgfra regulatory elements with the analysis of lineage-specific markers, we investigated the events leading to PrE and EPI lineage segregation in the mouse, and correlated our findings using an embryo staging system based on total cell number. Before blastocyst formation, lineage-specific factors are expressed in an overlapping manner. Subsequently, a gradual progression towards a mutually exclusive expression of PrE- and EPI-specific markers occurs. Finally, cell sorting is achieved by a variety of cell behaviours and by selective apoptosis.
Keywords: controlled study; protein expression; genetics; nonhuman; protein localization; protein analysis; animal cell; mouse; animal; cytology; metabolism; mammalia; animals; mice; cell function; platelet derived growth factor alpha receptor; receptor, platelet-derived growth factor alpha; apoptosis; biological model; models, biological; green fluorescent protein; embryo development; cell differentiation; cell population; cell lineage; regulatory mechanism; hybrid protein; recombinant fusion proteins; histone; reporter gene; green fluorescent proteins; pregnancy; cell count; down regulation; genes, reporter; histones; developmental stage; genetic marker; genetic markers; transcription factor nanog; cell selection; endoderm; live imaging; blastocyst; cells; cell; epiblast; primitive endoderm; transcription factor gata 4; inner cell mass; lineage specification; histone h2b-gfp fusion; icm; mouse blastocyst; pdgfrα
Journal Title: Development
Volume: 135
Issue: 18
ISSN: 0950-1991
Publisher: Company of Biologists  
Date Published: 2008-09-01
Start Page: 3081
End Page: 3091
Language: English
DOI: 10.1242/dev.021519
PUBMED: 18725515
PROVIDER: scopus
PMCID: PMC2768606
DOI/URL:
Notes: --- - "Cited By (since 1996): 56" - "Export Date: 17 November 2011" - "CODEN: DEVPE" - "Source: Scopus"
Altmetric Score
MSK Authors
  1. Berenika Anna Plusa
    2 Plusa
  2. Jerome Francois Artus
    14 Artus