DNA damage and repair capacity in patients with lung cancer: Prediction of multiple primary tumors Journal Article


Authors: Orlow, I.; Park, B. J.; Mujumdar, U.; Patel, H.; Siu-Lau, P.; Clas, B. A.; Downey, R.; Flores, R.; Bains, M.; Rizk, N.; Dominguez, G.; Jani, J.; Berwick, M.; Begg, C. B.; Kris, M. G.; Rusch, V. W.
Article Title: DNA damage and repair capacity in patients with lung cancer: Prediction of multiple primary tumors
Abstract: Purpose: Patients who survive one occurrence of non-small-cell lung cancer (NSCLC) are at higher risk of a second malignancy. Capacity to repair damaged DNA may modulate individual susceptibility to develop lung cancer. Therefore, we evaluated constitutive and induced DNA damage, and repair capacity, in patients with multiple NSCLCs (cases) and compared the results to those obtained in patients with a single NSCLC (controls). Patients and Methods: One hundred eight cases and 99 controls matched by age, sex, and time since diagnosis were studied. DNA damage was assessed on peripheral blood lymphocytes by the comet assay before and after exposing cells to a tobacco-derived carcinogen, using the tail moment and the tail intensity as measures to assess baseline damage, induced damage and repair capacity. Results: Constitutive DNA damage, benzo(a)pyrene diol epoxide-induced damage, and repair after BPDE-induced damage were all significantly higher in cases than in controls. These results were confirmed in regression analyses adjusted for potential confounders. Conclusion: DNA damage as measured by the comet assay is associated with the development of multiple primary tumors in individuals with NSCLC. © 2008 by American Society of Clinical Oncology.
Keywords: adult; controlled study; human tissue; aged; middle aged; human cell; major clinical study; case control study; genetics; case-control studies; histopathology; dna damage; dna repair; lung non small cell cancer; carcinoma, non-small-cell lung; lung neoplasms; pathology; lung tumor; dna; dna adduct; dna adducts; tobacco; multiple cancer; neoplasms, multiple primary; carcinogen; peripheral lymphocyte; carcinogens; comet assay; dihydroxybenzo[a]pyrene oxide; benzo[a]pyrene 7,8 dihydrodiol 9,10 oxide; 7,8-dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
Journal Title: Journal of Clinical Oncology
Volume: 26
Issue: 21
ISSN: 0732-183X
Publisher: American Society of Clinical Oncology  
Date Published: 2008-07-20
Start Page: 3560
End Page: 3566
Language: English
DOI: 10.1200/jco.2007.13.2654
PUBMED: 18640936
PROVIDER: scopus
PMCID: PMC4423737
DOI/URL:
Notes: --- - "Cited By (since 1996): 8" - "Export Date: 17 November 2011" - "CODEN: JCOND" - "Source: Scopus"
Altmetric Score
MSK Authors
  1. Colin B Begg
    234 Begg
  2. Valerie W Rusch
    652 Rusch
  3. Nabil Rizk
    134 Rizk
  4. Marianne Berwick
    119 Berwick
  5. Irene Orlow
    191 Orlow
  6. Raja Flores
    107 Flores
  7. Bernard J Park
    155 Park
  8. Robert J Downey
    202 Downey
  9. Manjit S Bains
    227 Bains
  10. Mark Kris
    597 Kris
  11. Himali S Patel
    8 Patel
  12. Jennifer Jani
    1 Jani
  13. Brian Clas
    9 Clas