The regulatory activity of microRNA* species has substantial influence on microRNA and 3′ UTR evolution Journal Article
| Authors: | Okamura, K.; Phillips, M. D.; Tyler, D. M.; Duan, H.; Chou, Y. T.; Lai, E. C. |
| Article Title: | The regulatory activity of microRNA* species has substantial influence on microRNA and 3′ UTR evolution |
| Abstract: | During microRNA (miRNA) biogenesis, one strand of a ∼21-22-nucleotide RNA duplex is preferentially selected for entry into a silencing complex. The other strand, known as the miRNA* species, has typically been assumed to be a carrier strand. Here we show that, although Drosophila melanogaster miRNA* species are less abundant than their partners, they are often present at physiologically relevant levels and can associate with Argonaute proteins. Comparative genomic analyses revealed that >40% of miRNA* sequences resist nucleotide divergence across Drosophilid evolution, and at least half of these well-conserved miRNA* species select for conserved 3′ untranslated region seed matches well above background noise. Finally, we validated the inhibitory activity of miRNA* species in both cultured cells and transgenic animals. These data broaden the reach of the miRNA regulatory network and suggest an important mechanism that diversifies miRNA function during evolution. © 2008 Nature Publishing Group. |
| Keywords: | controlled study; nonhuman; animals; microrna; embryo; gene function; evolution; transgenic animal; animalia; evolution, molecular; cell culture; immunoprecipitation; genomics; 3' untranslated region; drosophila melanogaster; nucleic acid conformation; micrornas; argonaute protein; 3' untranslated regions; animals, genetically modified; noise; drosophilidae |
| Journal Title: | Nature Structural and Molecular Biology |
| Volume: | 15 |
| Issue: | 4 |
| ISSN: | 1545-9993 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2008-04-01 |
| Start Page: | 354 |
| End Page: | 363 |
| Language: | English |
| DOI: | 10.1038/nsmb.1409 |
| PUBMED: | 18376413 |
| PROVIDER: | scopus |
| PMCID: | PMC2698667 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 102" - "Export Date: 17 November 2011" - "CODEN: NSMBC" - "Source: Scopus" |
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