Flow cytometric analysis of cytokinetics of L3-acute lymphoblastic leukemia/lymphoma Journal Article
| Authors: | Lichtman, S. M.; Andreeff, M. |
| Article Title: | Flow cytometric analysis of cytokinetics of L3-acute lymphoblastic leukemia/lymphoma |
| Abstract: | Cell kinetic differences have been described between acute lymphoblastic leukemia with L1 and L2 morphology. We now report cytokinetic and DNA ploidy findings of the rare L3 B-cell leukemia/lymphoma. Flow cytometry analysis of nineteen samples was performed by simultaneous DNA-RNA staining with acridine orange. RNA and DNA indices and cell cycle distributions were calculated. The RNA-index of the G0/G1 cells was 17.9 ± 8.7 and the number of cells in S phase and S + G2M were 21 ± 10.6 and 28.0 ± 13.9 percent respectively. DNA aneuploidy was found in 6/19 (31.6%) and in two cases multiple aneuploid cell lines were observed. DNA aneuploidy and multiple abnormal stemlines adversly affected survival (p < 0.05), while kinetic parameters did not affect survival (p > 0.05). The cytokinetic data are significantly different (S phase and RNA-I; p < 0.001) than previously reported for the L1 and L2 ALL. Abnormal DNA stemlines were found in cases with no detected cytogenetic abnormalities. This study confirms that L3 ALL is characterized by significantly increased proliferation and provides a means for a flow cytometric identification of this subtype as compared to L1 and L2 ALL. © 1992. |
| Keywords: | adult; cancer survival; clinical article; aged; retrospective studies; human cell; flow cytometry; mitosis; cell cycle; cell cycle s phase; acute lymphoblastic leukemia; rna; chromosome aberration; b cell lymphoma; dna; tumor cell line; dna, neoplasm; staining; cell cycle g2 phase; cancer classification; acridine orange; aneuploidy; lymphoma, aids-related; cell cycle g1 phase; burkitt lymphoma; cell cycle g0 phase; b cell leukemia; middle age; rna analysis; lymphoblastoma; cell kinetics; dna content; ploidy; human; male; female; priority journal; article; leukemia, b-cell, acute; cytokinetics; dna-index; l3 morphology; rna-index |
| Journal Title: | Leukemia Research |
| Volume: | 16 |
| Issue: | 9 |
| ISSN: | 0145-2126 |
| Publisher: | Elsevier Ltd |
| Date Published: | 1992-09-01 |
| Start Page: | 853 |
| End Page: | 859 |
| Language: | English |
| DOI: | 10.1016/0145-2126(92)90031-2 |
| PUBMED: | 1405716 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
