Escherichia coli topoisomerase III-catalyzed cleavage of RNA Journal Article
| Authors: | DiGate, R. J.; Marians, K. J. |
| Article Title: | Escherichia coli topoisomerase III-catalyzed cleavage of RNA |
| Abstract: | Relaxation of superhelical DNA by Escherichia coli topoisomerase III (Topo III) was inhibited by the inclusion of tRNA in the reaction mixture. Investigation of the basis of this inhibition revealed that Topo III could bind RNA and establish a cleavage-rehgation equilibrium. The addition of SDS to these reaction mixtures induced cleavage of the RNA by Topo III. The nucleotide sequences of RNA and DNA cleavage sites were identical, although cleavage site preference differed. Thus, the possibility that Topo III can pass strands of RNA as well as strands of DNA must be considered in accounting for the role of this enzyme in nucleic acid metabolism. |
| Keywords: | nonhuman; enzyme activity; rna; dna; molecular sequence data; kinetics; nucleotide sequence; escherichia coli; substrate specificity; base sequence; transfer rna; enzyme binding; sodium chloride; dna topoisomerase; dna topoisomerases, type i; nucleic acid metabolism; oligodeoxyribonucleotides; dna supercoiling; dna strand; oligoribonucleotides; priority journal; article; support, u.s. gov't, p.h.s. |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 267 |
| Issue: | 29 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1992-10-15 |
| Start Page: | 20532 |
| End Page: | 20535 |
| Language: | English |
| PUBMED: | 1383203 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
