Two classes of DNA end-joining reactions catalyzed by vaccinia topoisomerase I Journal Article
| Author: | Shuman, S. |
| Article Title: | Two classes of DNA end-joining reactions catalyzed by vaccinia topoisomerase I |
| Abstract: | The ability of a eukaryotic DNA topoisomerase I to catalyze DNA rearrangements was examined in vitro using defined substrates and purified enzyme. Site-specific DNA strand cleavage by vaccinia topoisomerase I across from a nick generated double-strand breaks that could be religated to a heterologous blunt-ended duplex DNA regardless of the sequence of the acceptor molecule. Topoisomerase bound covalently at internal positions could religate the bound strand to an incoming acceptor provided that DNA molecule had sequence homology to the region 3′ of the scissile bond. These end-joining reactions suggest two potential modes of topoisomerase-mediated recombination that differ in their requirements for DNA homology. |
| Keywords: | dna strand breakage; dna; double stranded dna; molecular sequence data; kinetics; eukaryota; substrate specificity; vaccinia virus; base sequence; plasmids; dna structure; catalysis; sequence homology; sequence homology, nucleic acid; enzyme binding; dna topoisomerase; dna topoisomerases, type i; vaccinia; virus dna; dna strand; virus enzyme; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 267 |
| Issue: | 24 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1992-08-25 |
| Start Page: | 16755 |
| End Page: | 16758 |
| Language: | English |
| PUBMED: | 1324909 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
