Increase in acridine orange (AO) fluorescence intensity of monocytes cultured in plastic tissue culture plates as measured by flow cytometry Journal Article


Authors: Myc, A.; Pizzolo, J. G.; Dygulski, K.; Melamed, M. R.
Article Title: Increase in acridine orange (AO) fluorescence intensity of monocytes cultured in plastic tissue culture plates as measured by flow cytometry
Abstract: Although the green‐red fluorescence of AO is an accepted measure of DNA‐RNA content, respectively, it is actually a measure of the fluorescence of dye bound to nucleic acids, and may vary with changes in accessibility to the dye. It has been shown for example that extraction of nuclear proteins results in a marked increase in DNA stainability. Moreover, in certain cell systems the binding of fluorochromes correlates with structural modifications in chromatin that accompany cell differentiation. We report here that changes in green & red fluorescence intensity also occur in long‐term monocyte cultures. The increased red fluorescence intensity observed in cultured monocytes may reflect ribosomal RNA synthesis and the increased green fluorescence enhanced AO accessibility to DNA due to changes in chromatin organization. We compared cultured monocytes from bladder cancer patients and healthy donors. The results indicate a small but statistically significantly greater increase in mean green & red fluorescence of cultured monocytes from the cancer patients. These fluorescence variations may indicate differences in the immunologic status of cancer patients and/or be related to disease state. Copyright © 1992 Wiley‐Liss, Inc.
Keywords: controlled study; human cell; comparative study; flow cytometry; cells, cultured; fluorescence; dna; cell culture; chromatin; bladder neoplasms; monocyte; monocytes; acridine orange; indoles; lymphocyte; lymphocytes; tissue culture; rna analysis; dna content; human; priority journal; article; support, u.s. gov't, p.h.s.; endogenous compound; dapi; chromatin organization; dna & rna content; stainability
Journal Title: Cytometry
Volume: 13
Issue: 1
ISSN: 0196-4763
Publisher: Wiley Liss  
Date Published: 1992-01-01
Start Page: 103
End Page: 107
Language: English
DOI: 10.1002/cyto.990130115
PUBMED: 1547653
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 30 July 2019 -- Source: Scopus
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  1. Myron Melamed
    148 Melamed