Abbott IMx® evaluated for assay of prostate-specific antigen in serum Journal Article


Authors: Dnistrian, A. M.; Schwartz, M. K.; Smith, C. A.; Nisselbaum, J. S.; Fair, W. R.
Article Title: Abbott IMx® evaluated for assay of prostate-specific antigen in serum
Abstract: We evaluated a new fully automated procedure for quantitative measurement of prostate-specific antigen (PSA) by the Microparticle Enzyme Immunoassay (MEIA) technology developed for the Abbott IMx® automated immunoassay system. The performance characteristics of the Abbott IMx PSA assay (y) were evaluated and compared with those of the Hybritech Tandem®-E PSA assay (x), a solid-phase two-site immunoenzymometric assay. PSA values for both assays were well correlated (r = 0.99); regression analysis yielded the equation y = 0.92x - 0.23 μg/L. The Abbott assay proved reliable and reproducible, as shown by the intra- and interassay coefficients of variation (2.0-3.4% and 3.1-4.7%, respectively). The assay gave a linear standard curve up to 100 μg/L and was very sensitive (detected PSA <0.1 μg/L). This analytical sensitivity was comparable with that of the Tandem-E PSA assay. Overall, the IMx PSA assay demonstrated the accuracy, precision, linearity, and intermethod correlation required for monitoring patients with prostate cancer.
Keywords: comparative study; reproducibility; prostate specific antigen; accuracy; patient monitoring; prostate cancer; prostate-specific antigen; standard; enzyme immunoassay; immunoenzyme techniques; blood; intermethod comparison; immunoassay; regression analysis; autoanalysis; reagent kits, diagnostic; analytical equipment; human; male; priority journal; article
Journal Title: Clinical Chemistry
Volume: 38
Issue: 10
ISSN: 0009-9147
Publisher: American Association for Clinical Chemistry  
Date Published: 1992-10-01
Start Page: 2140
End Page: 2142
Language: English
PUBMED: 1382898
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 30 July 2019 -- Source: Scopus
Citation Impact
MSK Authors
  1. William R Fair
    342 Fair
  2. Morton Schwartz
    186 Schwartz
  3. Carol   Smith
    39 Smith