| Abstract: |
Some human squamous cell carcinomas contain DNA of human papillomaviruses (HPV) and express RNA from the E6 and E7 genes. We have examined the effect of plasmids that express antisense RNA of these genes on the growth of the human cancer cell lines HeLa, C4-1, and 1483, which contain HPV type 18 DNA. As controls, the human cancer cell line 183 and the Vero line of monkey kidney cells were used, which do not contain HPV. Plasmids were introduced into the cells by electroporation; cells that contained HPV type 18 accepted the anti-sense-expressing plasmids at a lower frequency than the cells that lacked HPV. Cell lines were developed from HeLa cells that contained sense- or antisense-expressing plasmids, and lines that contained anti-sense-expressing plasmids showed slower growth, reduced ability to form colonies in soft agar, and increased serum requirements. The use of antisense HPV RNA might be a suitable approach to gene therapy of HPV-expressing human cancers. © 1992, American Association for Cancer Research. All rights reserved. |
| Keywords: |
human cell; squamous cell carcinoma; nonhuman; animal cell; animal; cell division; gene expression; cell growth; cancer cell culture; tumor cells, cultured; hela cell; hela cells; transfection; genetic transfection; cell culture; rna, messenger; cancer cell; plasmid; gene therapy; electroporation; plasmids; cell adhesion; rna, viral; cell transformation, viral; vero cell; vero cells; complementary rna; papilloma virus; rna, antisense; papillomavirus; human; priority journal; article
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