Three-drug synergistic inhibition of HIV-1 replication in vitro by 3′-fluoro-3′-deoxythymidine, recombinant soluble CD4, and recombinant interferon-alpha Journal Article

  

Authors:	Pan, X. Z.; Qiu, Z. D.; Baron, P. A.; Gold, J. W. M.; Polsky, B.; Chou, T. C.; Armstrong, D.
Article Title:	Three-drug synergistic inhibition of HIV-1 replication in vitro by 3′-fluoro-3′-deoxythymidine, recombinant soluble CD4, and recombinant interferon-alpha
Abstract:	3′-Fluoro-3′deoxythymidine (FLT), recombinant soluble CD4 (CD4), and recombinant interferon-alpha (IFNα) were evaluated in two- and three-drug regimens against HIV-1 replication in vitro. Peripheral blood mononuclear cells were studied using p24 antigen production as the virologic endpoints. FLT showed 2.5-fold higher efficacy and a similar selectivity index to zidovudine. Drug interactions were evaluated by the median effect principle and the isobologram technique. FLT, CD4, and interferon alpha at noncytotoxic concentrations inhibited HIV-1 synergistically in two- and three-drug combinations with a combination index smaller than one and dose reduction index greater than one. The three-drug regimen provided greater virus suppression than the two-drug regimen. These results suggest that FLT is an alternative agent to AZT for the treatment of HIV infection either as a single agent or in combination with CD4 and/or interferon-alpha. © 1992, Mary Ann Liebert, Inc. All rights reserved.
Keywords:	controlled study; human cell; drug potentiation; nonhuman; human immunodeficiency virus infection; cells, cultured; drug synergism; recombinant proteins; cd4 antigen; virus replication; antigens, cd4; human immunodeficiency virus 1; interferon-alpha; hiv-1; zidovudine; antiviral agents; solubility; virus inhibition; dideoxynucleosides; 3' fluorothymidine; recombinant alpha interferon; human; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
Journal Title:	AIDS Research and Human Retroviruses
Volume:	8
Issue:	5
ISSN:	0889-2229
Publisher:	Mary Ann Liebert, Inc
Date Published:	1992-05-01
Start Page:	589
End Page:	595
Language:	English
DOI:	10.1089/aid.1992.8.589
PUBMED:	1515212
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026992106&doi=10.1089%2faid.1992.8.589&partnerID=40&md5=cfb64ca1ecd8a6b6974812c86a7f23bb
Notes:	Article -- Export Date: 30 July 2019 -- Source: Scopus

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