The maternal to zygotic transition regulates genome-wide heterochromatin establishment in the zebrafish embryo Journal Article
| Authors: | Laue, K.; Rajshekar, S.; Courtney, A. J.; Lewis, Z. A.; Goll, M. G. |
| Article Title: | The maternal to zygotic transition regulates genome-wide heterochromatin establishment in the zebrafish embryo |
| Abstract: | The segregation of eukaryotic genomes into euchromatin and heterochromatin represents a fundamental and poorly understood process. Here, we demonstrate that genome-wide establishment of heterochromatin is triggered by the maternal to zygotic transition (MZT) during zebrafish embryogenesis. We find that prior to MZT, zebrafish lack hallmarks of heterochromatin including histone H3 lysine 9 trimethylation (H3K9me3) and condensed chromatin ultrastructure. Global establishment of heterochromatic features occurs following MZT and requires both activation of the zygotic genome and degradation of maternally deposited RNA. Mechanistically, we demonstrate that zygotic transcription of the micro RNA miR-430 promotes degradation of maternal RNA encoding the chromatin remodeling protein Smarca2, and that clearance of Smarca2 is required for global heterochromatin establishment in the early embryo. Our results identify MZT as a key developmental regulator of heterochromatin establishment during vertebrate embryogenesis and uncover functions for Smarca2 in protecting the embryonic genome against heterochromatinization. © 2019, The Author(s). |
| Keywords: | immunohistochemistry; unclassified drug; gene sequence; nonhuman; polymerase chain reaction; microrna; genome-wide association study; embryo development; genetic transcription; vertebrata; eukaryota; histone h3; western blotting; immunoprecipitation; real time polymerase chain reaction; rna polymerase ii; ultrastructure; lysine; zebra fish; rna extraction; transposon; heterochromatin; chromatin assembly and disassembly; danio rerio; microinjection; rna degradation; transmission electron microscopy; chromatin condensation; zygote; brm protein; gene knockdown; article; morpholino oligonucleotide; micro rna 430; histone h3 lysine 9 trimethylation |
| Journal Title: | Nature Communications |
| Volume: | 10 |
| ISSN: | 2041-1723 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2019-04-04 |
| Start Page: | 1551 |
| Language: | English |
| DOI: | 10.1038/s41467-019-09582-3 |
| PUBMED: | 30948728 |
| PROVIDER: | scopus |
| PMCID: | PMC6449393 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 May 2019 -- Source: Scopus |
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