Hexamethylenebisacetamide-induced erythroleukemia cell differentiation involves modulation of events required for cell cycle progression through G(1) Journal Article

   


Authors: Kiyokawa, H.; Richon, V. M.; Venta-Perez, G.; Rifkind, R. A.; Marks, P. A.
Article Title: Hexamethylenebisacetamide-induced erythroleukemia cell differentiation involves modulation of events required for cell cycle progression through G(1)
Abstract: Hexamethylenebisacetamide (HMBA), a potent inducer of differentiation of transformed cells such as murine erythroleukemia cells, causes a prolongation of the G1 phase of the cell cycle during which commitment to terminal differentiation is first detected. Removal of HMBA prior to the G1 phase aborts commitment. To further define the relationship between the G1 phase and commitment to differentiation, we used two inhibitors of cell cycle progression: aphidicolin, which blocks cells at the G1/S interphase, and deferoxamine, which blocks cells at an earlier stage during G1. HMBA- induced prolongation of G1 is associated with the accumulation of underphosphorylated retinoblastoma protein, decrease in cyclin A protein levels, and commitment to differentiation. G1 arrest of murine erythroleukemia cells induced by aphidicolin or deferoxamine is not associated with accumulation of underphosphorylated retinoblastoma protein, suppression of cyclin A protein, or commitment of cells to terminal differentiation. Neither of the cell cycle inhibitors alters the effect of HMBA in inducing the G1-associated changes or commitment to differentiation. Taken together, the present findings indicate that the site of action of HMBA which leads to commitment is in a stage of the G1 phase prior to the point of cell cycle block caused by deferoxamine or aphidicolin. HMBA appears to cause cell differentiation with suppression of cell cycle progression by an action that affects events required for cell progression through G1, including accumulation of underphosphorylated retinoblastoma protein and changes in regulation of cyclin levels.
Keywords: controlled study; protein phosphorylation; nonhuman; animal cell; mouse; cell cycle s phase; cell differentiation; retinoblastoma; animalia; cell transformation; murinae; cyclin a; cell cycle g1 phase; deferoxamine; hexamethylenebisacetamide; priority journal; article; erythroleukemia cell; aphidicolin
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 90
Issue: 14
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1993-07-15
Start Page: 6746
End Page: 6750
Language: English
DOI: 10.1073/pnas.90.14.6746
PUBMED: 8341693
PROVIDER: scopus
PMCID: PMC47009
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027284241&doi=10.1073%2fpnas.90.14.6746&partnerID=40&md5=798ff58f856744f1d0c4ea3b16299e26
Notes: Article -- Export Date: 1 March 2019 -- Source: Scopus
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MSK Authors
  1. Victoria M Richon
    91 Richon
  2. Gisela Venta-Perez
    23 Venta-Perez
  3. Paul Marks
    186 Marks
  4. Richard Rifkind
    118 Rifkind
  5. Hiroaki Kiyokawa
    27 Kiyokawa
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