Interferon γ-induced transcription of the high-affinity Fc receptor for IgG requires assembly of a complex that includes the 91-kDa subunit of transcription factor ISGF3 Journal Article
| Authors: | Pearse, R. N.; Feinman, R.; Shuai, K.; Darnell, J. E. Jr; Ravetch, J. V. |
| Article Title: | Interferon γ-induced transcription of the high-affinity Fc receptor for IgG requires assembly of a complex that includes the 91-kDa subunit of transcription factor ISGF3 |
| Abstract: | A 39-nt DNA sequence, the interferon γ (IFN-γ) response region (GRR), is necessary for the IFN-γ-induced transcription of the high-affinity Fc receptor for IgG (FcγRI) and sufficient for the IFN-γ-induced transcription of transfected plasmids. By using extracts from IFN-γ-treated cells, three protein complexes will assemble in vitro on a 9-nt core region in the 3' domain of the GRR. The sequence of this core resembles the IFN-γ-activated sequence (GAS) described for the GBP gene. Mutations in this GAS core region prevent complex assembly and result in the loss of IFN-γ induction of reporter constructs containing the mutation. In addition to the GAS core region, a 5' region of the GRR is necessary for optimal IFN-γ induction and for formation of one of the DNA-protein complexes. By antibody reactivity, we show that a 91-kDa protein, first identified as a component of ISGF3, the IFN-γ-induced transcription complex, is present in at least two of the DNA- protein complexes. IFN-α can induce the formation of the faster-migrating 91-kDa protein-GAS complex but not the slower-migrating complex. Furthermore, IFN-α does not result in appreciable transcriptional activation of FcγRI or constructs containing the GRR. Thus, these data demonstrate that the IFN-γ- activated 91-kDa protein is required for IFN-γ induction of FcγRI and suggest that an additional complex may be required for optimal expression and specificity. |
| Keywords: | dna binding protein; human cell; protein analysis; protein dna binding; transcription factor; transcription regulation; gamma interferon; immunoglobulin g; protein induction; fc receptor; dna sequence; gene induction; dna protein complex; human; priority journal; article |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 90 |
| Issue: | 9 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1993-05-01 |
| Start Page: | 4314 |
| End Page: | 4318 |
| Language: | English |
| DOI: | 10.1073/pnas.90.9.4314 |
| PUBMED: | 8483949 |
| PROVIDER: | scopus |
| PMCID: | PMC46497 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 March 2019 -- Source: Scopus |
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