Oligodeoxynucleotides containing 2'-fluoro-arabino-nucleotide and C-nucleotide Conference Paper
| Authors: | Kois, P.; Watanabe, K. A. |
| Title: | Oligodeoxynucleotides containing 2'-fluoro-arabino-nucleotide and C-nucleotide |
| Conference Title: | 20th Symposium on Nucleic Acids Chemistry |
| Abstract: | Oligonucleotides containing 2'-fluoro-ara-pyrimidines and 1-methyl-2'-deoxy-psi-uridine were synthesized. The melting profiles show that the presence of FMAU has a large stabilizing effect on the duplex. Replacement of dT with FAU or psi-dT, or dC with FAC resulted in the formation of less stable duplexes. Temperature-dependent CD spectroscopy demonstrated that the structures of these oligomers are very similar to those of unmodified oligomers, however, these minor alterations in structure of dodecamers resulted in faster splitting by endonuclease EcoR1. |
| Keywords: | molecular genetics; chemistry; molecular sequence data; nucleotide sequence; drug derivative; uracil arabinoside; arabinofuranosyluracil; restriction endonuclease; base sequence; thermodynamics; hydrolysis; oligodeoxyribonucleotides; circular dichroism; article; dna restriction enzymes; oligodeoxyribonucleotide |
| Journal Title | Nucleic Acids Symposium Series |
| Volume: | 29 |
| Issue: | 29 |
| Conference Dates: | 1993 Nov 9-11 |
| Conference Location: | Sapporo, Japan |
| ISBN: | 0261-3166 |
| Publisher: | Oxford University Press |
| Date Published: | 1993-01-01 |
| Start Page: | 215 |
| End Page: | 216 |
| Language: | English |
| PUBMED: | 8247773 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 March 2019 -- Source: Scopus |
