Ex vivo expansion and gene therapy using cord blood CD34+ cells Journal Article
| Author: | Moore, M. A. S. |
| Article Title: | Ex vivo expansion and gene therapy using cord blood CD34+ cells |
| Abstract: | CD34+ cells can be efficiently isolated from cord blood by sequential Percoll, Ficoll—Hypaque, and immunomagnetic bead separation. This population, containing up to 25% progenitor cells and 0.2-1% stem cells, defined by a long-term culture-initiating cell (LTC-initiating cell) assay, can be expanded in vitro in the presence of a specific combination of cytokines resulting in a >100,000-fold increase in cells, a >2,000-fold increase in progenitors, and an 18- to 20-fold increase in LTC-initiating cells. These latter populations can be efficiently transfected with a retroviral vector expressing a mutated dehydrofolate-resistance gene that confers methotrexate reductase. © 1993, Mary Ann Liebert, Inc. All rights reserved. |
| Keywords: | adult; human cell; conference paper; methotrexate; cells, cultured; cd34 antigen; stem cell factor; erythropoietin; hematopoietic stem cell transplantation; drug resistance; genetic vectors; stem cell; cytokine; cytokines; fetal blood; immunotherapy; recombinant fusion proteins; cell culture; gene therapy; hematopoietic stem cells; interleukin 6; umbilical cord blood; fetus; bone marrow cell; antigens, cd; dihydrofolate reductase; tetrahydrofolate dehydrogenase; cell separation; antigens, cd34; retrovirus; retroviridae; colony forming unit gm; virus protein; virus vector; interleukin 1; colony formation; interleukin 3; immunogenetics; burst forming unit e; colony forming unit gemm; human; priority journal |
| Journal Title: | Journal of Hematotherapy |
| Volume: | 2 |
| Issue: | 2 |
| ISSN: | 1061-6128 |
| Publisher: | Mary Ann Liebert, Inc |
| Date Published: | 1993-01-01 |
| Start Page: | 221 |
| End Page: | 224 |
| Language: | English |
| DOI: | 10.1089/scd.1.1993.2.221 |
| PUBMED: | 7522878 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 March 2019 -- Source: Scopus |
