Comparison of antigen expression on fresh and cultured ascites cells and on solid tumors of patients with epithelial ovarian cancer Journal Article


Authors: Provencher, D. M.; Finstad, C. L.; Saigo, P. E.; Rubin, S. C.; Hoskins, W. J.; Federici, M. G.; Stockert, E.; Lloyd, K. O.; Lewis, J. L. Jr
Article Title: Comparison of antigen expression on fresh and cultured ascites cells and on solid tumors of patients with epithelial ovarian cancer
Abstract: The antigenic phenotype of malignant cells from ascites of patients with epithelial ovarian cancers was examined and compared to that of their primary and metastatic sites. Cell-surface antigens on frozen sections of primary and metastatic tumors and frozen cell pellets from ascites were analyzed with a panel of murine monoclonal antibodies using the indirect immunoperoxidase method. In addition, ascites cells cultured with and without autologous cell- free ascitic fluid were evaluated by immunofluorescence. The pattern of antigen expression detected on fresh and cultured ascitic epithelial cells was shown to be identical to the expression in autologous solid tumor tissues. When placed in culture, malignant epithelial cells generally persisted for a minimum of one, but no more than five passages. Addition of autologous ascitic fluid to cultures of ascites cells did not alter the phenotype of the epithelial tumor cell population and did not enhance the growth of these cells. From one culture of ascites cells a permanent malignant epithelial ovarian cancer cell line (designated SK-OV-8) was established. The demonstration that epithelial tumor cells found in ascites of patients with epithelial ovarian cancer have the identical antigenic phenotype as their solid tumor counterpart, at least for the panel of antigens studied, may be useful in planning imaging and therapeutic trials with monoclonal antibodies. © 1993 Academic Press, Inc.
Journal Title: Gynecologic Oncology
Volume: 50
Issue: 1
ISSN: 0090-8258
Publisher: Elsevier Inc.  
Date Published: 1993-07-01
Start Page: 78
End Page: 83
Language: English
DOI: 10.1006/gyno.1993.1167
PROVIDER: scopus
PUBMED: 8349167
DOI/URL:
Notes: Source: Scopus
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MSK Authors
  1. William Hoskins
    194 Hoskins
  2. Kenneth O Lloyd
    94 Lloyd
  3. Patricia E Saigo
    60 Saigo
  4. John   Lewis
    57 Lewis
  5. Connie L. Finstad
    28 Finstad
  6. Stephen C. Rubin
    53 Rubin