Sporadic amplification of the MYC gene in human osteosarcomas Journal Article
| Authors: | Ladanyi, M.; Park, C. K.; Lewis, R.; Jhanwar, S. C.; Healey, J. H.; Huvos, A. G. |
| Article Title: | Sporadic amplification of the MYC gene in human osteosarcomas |
| Abstract: | The MYC proto-oncogene has been shown to be overex-pressed in several types of sarcomas, including some osteosarcomas. In most cases, the overexpression is due to gene amplification. The total number of osteosarcoma patients studied, however, remains too small to derive any conclusions regarding the true prevalence and the possible clinical significance of MYC gene amplification. To address the issue more thoroughly, we studied 27 specimens from 25 patients with high-grade osteosarcoma (16 primary, 11 metastatic; 11 adult. 14 pediatric) for MYC gene alterations by Southern blot analysis. Two of 27 specimens (19c) showed MYC gene amplification: A primary fibrohistiocytic osteosarcoma of the femur in a 37-year-old man showed threefold amplification, and a primary Paget's osteosarcoma of the tibia in a 60-year-old man showed fourfold amplification. None of the specimens tested showed MYC gene rearrangement (zero of 27) or activating point mutations at the Pvu II site in MYC exon-1 (zero of 26). Hence, the MYC gene is amplified in a subset of osteosarcomas. The possible clinical or biological significance of MYC gene amplification in osteosarcoma may warrant further investigation. © 1993 Raven-press. Ltd. |
| Keywords: | osteosarcoma; adult; child; clinical article; human tissue; bone neoplasms; proto oncogene; gene amplification; gene rearrangement; genes, myc; amplification; point mutation; oncogene myc; myc; middle age; southern blotting; proto-oncogene; blotting, southern; human; male; female; article; support, non-u.s. gov't |
| Journal Title: | Diagnostic Molecular Pathology |
| Volume: | 2 |
| Issue: | 3 |
| ISSN: | 1052-9551 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 1993-09-01 |
| Start Page: | 163 |
| End Page: | 167 |
| Language: | English |
| DOI: | 10.1097/00019606-199309000-00004 |
| PUBMED: | 8287230 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
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