Presence of point mutations in the N-ras gene in radiation-transformed rat embryo cells Journal Article
| Authors: | Chen, C. H.; Endlich, B.; Salavati, R.; Ling, C. C. |
| Article Title: | Presence of point mutations in the N-ras gene in radiation-transformed rat embryo cells |
| Abstract: | We study the transforming ability of X-rays in multistep carcinogenesis by irradiating primary rat cells which contain transfected c-myc oncogene. X-irradiation induces fully transformed phenotypes, including anchorage-independent growth and tumor formation in nude mice. Of seven foci examined, five exhibited an A to G conversion in codon 61 of the N-ras oncogene. Another transformed isolate has a single G to A base substitution in codon 14 in the same oncogene, while no point mutation is detected in the other focus. This is the first in vitro demonstration of the association between point mutation and X-ray-transformed cells. © 1993, American Association for Cancer Research. All rights reserved. |
| Keywords: | gene mutation; exons; nonhuman; phenotype; animal; mice; cell survival; embryo; transfection; carcinogenesis; gene activation; molecular sequence data; genetic transfection; cell transformation; genes, myc; irradiation; cell line, transformed; base sequence; base pairing; rats; dna mutational analysis; genes, ras; codon; point mutation; oncogene ras; oncogene myc; 3t3 cells; embryo cell; blotting, southern; priority journal; article; support, u.s. gov't, non-p.h.s. |
| Journal Title: | Cancer Research |
| Volume: | 53 |
| Issue: | 7 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1993-04-01 |
| Start Page: | 1511 |
| End Page: | 1515 |
| Language: | English |
| PUBMED: | 8453615 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
