Abnormal rectal cell proliferation and p52/p35 protein expression in patients with ulcerative colitis Journal Article
| Authors: | Paganelli, G. M.; Higgins, P. J.; Biasco, G.; Lipkin, M.; Brandi, G.; Santucci, R.; Miglioli, M.; Barbara, L. |
| Article Title: | Abnormal rectal cell proliferation and p52/p35 protein expression in patients with ulcerative colitis |
| Abstract: | We evaluated the presence of cell proliferation and antigenic abnormalities in rectal biopsies from 37 patients affected by ulcerative colitis and 15 controls. The study was carried out by thymidine labeling and immunochemistry, using antibodies against specific cytoskeletal-associated proteins (p52, p35, alpha-actinin). Among ulcerative colitis patients, 24 had an immunofluorescence pattern similar to that of controls, while 13 showed an abnormal distribution of one or more proteins (p52 alone or p52 and either p35 or alpha-actinin) within the rectal crypts. Patients showed a shift of the proliferative compartment towards the top of the rectal crypts compared with controls. This finding was more evident in patients with p52 or p35 abnormalities. Proliferative and antigenic defects were not related either to age or the duration of colitis. These phenotypic changes might be a biomarker of increased risk of colon cancer in ulcerative colitis. © 1993. |
| Keywords: | immunohistochemistry; adult; clinical article; controlled study; human tissue; aged; human cell; nonhuman; colorectal cancer; cell proliferation; biological marker; biological markers; phenotype; cell division; protein; immunofluorescence; colorectal neoplasms; antigen; colon cancer; protein induction; disease duration; ulcerative colitis; antibody; autoradiography; thymidine; immunochemistry; cytoskeleton protein; rectum; cytoskeletal proteins; middle age; antibody labeling; rectum biopsy; crypt cell; colitis, ulcerative; antigenic variation; human; male; female; priority journal; article; support, non-u.s. gov't; alpha actinin; cytoskeletal-associated antigens; rabbit antiserum |
| Journal Title: | Cancer Letters |
| Volume: | 73 |
| Issue: | 1 |
| ISSN: | 0304-3835 |
| Publisher: | Elsevier Ireland Ltd. |
| Date Published: | 1993-09-15 |
| Start Page: | 23 |
| End Page: | 28 |
| Language: | English |
| DOI: | 10.1016/0304-3835(93)90183-a |
| PUBMED: | 8402594 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
