Purification and analysis of a human sarcoma associated antigen Journal Article
| Authors: | Gupta, N. K.; Hirshaut, Y.; Schmall, B.; Feit, C. |
| Article Title: | Purification and analysis of a human sarcoma associated antigen |
| Abstract: | S1, a heterophile antigen present on human sarcoma cell lines in culture, has been previously defined by this laboratory [1,2]. This antigen is also present in guinea-pig kidney. Purification of the antigen to homogeneity has now been achieved by a combination of ammonium sulfate fractionation, DEAE-cellulose, sephadex, high pressure liquid chromotography and affinity chromotography. S1 is a monomeric protein of 70 000 Da, as indicated by the presence of a single band on SDS-PAGE. Amino acid analysis demonstrates the prevalence of glycine, lysine and glutamic acid. Aspartic acid was found to be the N-terminal residue with further sequence of glycine-valine-alanine-glutamic acid (gly-val-ala-glut). © 1993. |
| Keywords: | human cell; nonhuman; animal; animal tissue; neoplasm proteins; tumor antigen; animalia; sarcoma; kidney; antigen; amino terminal sequence; antigens, neoplasm; tumor cell line; amino acids; glutamic acid; molecular weight; aspartic acid; lysine; glycine; polyacrylamide gel electrophoresis; electrophoresis, polyacrylamide gel; amino acid analysis; purification; guinea pig; guinea pigs; antigen purification; human; priority journal; article; sus scrofa; cavia porcellus; sarcoma antigen; electrophoresis, disc |
| Journal Title: | Cancer Letters |
| Volume: | 69 |
| Issue: | 3 |
| ISSN: | 0304-3835 |
| Publisher: | Elsevier Ireland Ltd. |
| Date Published: | 1993-05-14 |
| Start Page: | 173 |
| End Page: | 180 |
| Language: | English |
| DOI: | 10.1016/0304-3835(93)90171-5 |
| PUBMED: | 8513443 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
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