Toward a prostate specific antigen-based prostate cancer diagnostic assay: Preparation of keyhole limpet hemocyanin-conjugated normal and transformed prostate specific antigen fragments Journal Article

   

Authors:	Dudkin, V. Y.; Miller, J. S.; Dudkina, A. S.; Antczak, C.; Scheinberg, D. A.; Danishefsky, S. J.
Article Title:	Toward a prostate specific antigen-based prostate cancer diagnostic assay: Preparation of keyhole limpet hemocyanin-conjugated normal and transformed prostate specific antigen fragments
Abstract:	Prostate specific antigen (PSA) molecules secreted by cancerous and normal prostate cells differ in their N-linked glycan composition, while the peptide backbone appears to be conserved. Antibodies selectively recognizing such differentially glycosylated PSA structures could form a basis for a new diagnostic assay for prostate cancer. Twenty-amino acid PSA fragments carrying di-, tri-, and tetrabranched complex-type glycans were prepared by total synthesis and conjugated to maleimide-modified keyhole limpet hemocyanin (KLH) carrier protein through backbone Cys residues. These glycopeptide/KLH conjugates were then used for antibody generation. © 2008 American Chemical Society.
Keywords:	cancer diagnosis; prostate specific antigen; peptide; assay; prostate cancer; prostate-specific antigen; prostatic neoplasms; amines; antigens; molecular recognition; carrier proteins; peptide fragments; glycosylation; antibodies; amino acids; organic acids; peeling; chemical reaction; synthesis; total synthesis; carbohydrate sequence; glycopeptide; cysteine; glycosylated; keyhole limpet hemocyanin; complexation; hemocyanin; maleimide; carbohydrate conformation; glycan; complex-type glycans; cys residues; n-linked glycan; peptide backbones; prostate cells
Journal Title:	Journal of the American Chemical Society
Volume:	130
Issue:	41
ISSN:	0002-7863
Publisher:	American Chemical Society
Date Published:	2008-10-15
Start Page:	13598
End Page:	13607
Language:	English
DOI:	10.1021/ja8028137
PUBMED:	18798614
PROVIDER:	scopus
PMCID:	PMC2646745
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-53849104657&partnerID=40&md5=419677552c193e8f68440719079f4871
Notes:	--- - "Cited By (since 1996): 6" - "Export Date: 17 November 2011" - "CODEN: JACSA" - "Source: Scopus"

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