Reptin and Pontin function antagonistically with PcG and TrxG complexes to mediate Hox gene control Journal Article
| Authors: | Diop, S. B.; Bertaux, K.; Vasanthi, D.; Sarkeshik, A.; Goirand, B.; Aragnol, D.; Tolwinski, N. S.; Cole, M. D.; Pradel, J.; Yates, J. R. 3rd; Mishra, R. K.; Graba, Y.; Saurin, A. J. |
| Article Title: | Reptin and Pontin function antagonistically with PcG and TrxG complexes to mediate Hox gene control |
| Abstract: | Pontin (Pont) and Reptin (Rept) are paralogous ATPases that are evolutionarily conserved from yeast to human. They are recruited in multiprotein complexes that function in various aspects of DNA metabolism. They are essential for viability and have antagonistic roles in tissue growth, cell signalling and regulation of the tumour metastasis suppressor gene, KAI1, indicating that the balance of Pont and Rept regulates epigenetic programmes critical for development and cancer progression. Here, we describe Pont and Rept as antagonistic mediators of Drosophila Hox gene transcription, functioning with Polycomb group (PcG) and Trithorax group proteins to maintain correct patterns of expression. We show that Rept is a component of the PRC1 PcG complex, whereas Pont purifies with the Brahma complex. Furthermore, the enzymatic functions of Rept and Pont are indispensable for maintaining Hox gene expression states, highlighting the importance of these two antagonistic factors in transcriptional output. |
| Keywords: | unclassified drug; mutation; nonhuman; protein function; animals; cell cycle proteins; chromosomal proteins, non-histone; protein binding; drosophila; gene function; genetic transcription; homeodomain proteins; gene expression regulation; transcription regulation; carrier proteins; trans-activators; drosophila melanogaster; gene silencing; adenosine triphosphatase; repressor proteins; drosophila proteins; genetic epigenesis; enzyme purification; dna helicases; drosophila protein; hox gene; cd82 antigen; embryonic ectoderm development protein; pontin; reptin; trithorax group protein |
| Journal Title: | EMBO Reports |
| Volume: | 9 |
| Issue: | 3 |
| ISSN: | 1469-221X |
| Publisher: | Wiley Blackwell |
| Date Published: | 2008-03-01 |
| Start Page: | 260 |
| End Page: | 266 |
| Language: | English |
| DOI: | 10.1038/embor.2008.8 |
| PUBMED: | 18259215 |
| PROVIDER: | scopus |
| PMCID: | PMC2267392 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 16" - "Export Date: 17 November 2011" - "CODEN: ERMEA" - "Source: Scopus" |
