Expression of P53, P27 and KI-67 in colorectal cancer patients of various ethnic origins: Clinical and tissue microarray based analysis Journal Article
| Authors: | Cohen, T.; Prus, D.; Shia, J.; Abu-Wasel, B.; Pinto, M. G.; Freund, H. R.; Stojadinovic, A.; Grakov, A.; Peretz, T.; Nissan, A. |
| Article Title: | Expression of P53, P27 and KI-67 in colorectal cancer patients of various ethnic origins: Clinical and tissue microarray based analysis |
| Abstract: | Background: This study was conducted to determine survival according to the expression of molecular markers in colorectal cancer (CRC) patients of various ethnic origins. Methods: Resection of primary tumor was conducted on 171 patients with CRC. Corresponding archived paraffin-embedded blocks were retrieved and tissue microarray (TMA) constructed. Immunohistochemical staining of the TMA for p53, p27 and Ki-67 was quantified by two independent pathologists. Survival was analyzed using the Kaplan-Meier product limit method. Results: With a median follow-up of 65 months, 56 patients (32.7%) died of disease. AJCC stage correlated with disease-free (DFS, P < 0.0001) and overall survival (OS, P < 0.0001). IHC staining was positive for Ki-67 in 77.4%, p53 in 55.8% and p27 in 54.2% of patients. Primary tumor marker expression did not correlate with DFS or OS. The 5-year DFS for Ashkenazi Jews was 75%, significantly higher than Sephardic Jews (SJ) 64% and Palestinian Arabs (PA) 38%, P = 0.001. Conclusions: Ethnicity among Ashkenazi and SJ and PA appears to have a significant impact on disease outcome in patients with CRC patients, while primary tumor expression of p53, p27 and Ki-67 was unrelated to disease outcome. |
| Keywords: | immunohistochemistry; adult; cancer survival; controlled study; human tissue; protein expression; disease-free survival; middle aged; protein array analysis; cancer surgery; major clinical study; cancer patient; cancer staging; follow up; follow-up studies; cancer diagnosis; colorectal cancer; adenocarcinoma; ki 67 antigen; ki-67 antigen; neoplasm recurrence, local; tumor markers, biological; age factors; colorectal neoplasms; statistical significance; ki-67; cyclin dependent kinase inhibitor 1b; protein p27; cyclin-dependent kinase inhibitor p27; tumor suppressor protein p53; multivariate analysis; tissue microarray; kaplan meier method; microarray; jews; ethnicity; jew; p53; p21; paraffin; elective surgery; arab; arabs |
| Journal Title: | Journal of Surgical Oncology |
| Volume: | 97 |
| Issue: | 5 |
| ISSN: | 0022-4790 |
| Publisher: | Wiley Blackwell |
| Date Published: | 2008-04-01 |
| Start Page: | 416 |
| End Page: | 422 |
| Language: | English |
| DOI: | 10.1002/jso.20989 |
| PUBMED: | 18286523 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 6" - "Export Date: 17 November 2011" - "CODEN: JSONA" - "Source: Scopus" |
