Combined Edman-chemical and laser-desorption mass spectrometric approaches to micro peptide sequencing: Optimization and applications Conference Paper

  

Authors:	Geromanos, S.; Casteels, P.; Elicone, C.; Powell, M.; Tempst, P.
Title:	Combined Edman-chemical and laser-desorption mass spectrometric approaches to micro peptide sequencing: Optimization and applications
Conference Title:	7th Symposium of the Protein Society
Abstract:	This chapter describes the optimization and application of combined Edman-chemical and laser-desorption mass spectrometric approaches to micro peptide sequencing. Transactions among membrane-bound compartments in the cytoplasm of eukaryotic cells are generally executed by transport vesicles that bud from one membrane and fuse selectively with another. Peptide fractions T11 and T12 yielded two and three peak m/z values, respectively, when analyzed by MALDI-MS, and the results indicated overlapping peptide species to be present in both fractions, likely because of cross-contamination after high-performance liquid chromatography. Unfortunately, although the sequencer-instrument was judged to be in perfect working condition, only a snippet of sequence was obtained for T12 and T11 failed to give any sequence at all. One of the three m/z values for T12 was in excellent agreement with the value for the predicted tryptic peptide containing the limited sequence, so confirming the identity of the entire peptide. © 1994, ACADEMIC PRESS, INC.
Journal Title	Techniques in Protein Chemistry
Volume:	5
Conference Dates:	1993 Jul 24-28
Conference Location:	San Diego, CA
ISBN:	1080-8914
Publisher:	Academic Press, Inc
Date Published:	1994-01-01
Start Page:	143
End Page:	150
Language:	English
DOI:	10.1016/b978-0-12-194710-1.50021-7
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013554753&doi=10.1016%2fB978-0-12-194710-1.50021-7&partnerID=40&md5=377e9eb69de0477bd06e846c05ffcf54
Notes:	Book Chapter (ISBN: 9780121947101) -- Export Date: 14 January 2019 -- Source: Scopus

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