Ex vivo expansion of cord blood-derived stem cells and progenitors Journal Article

  


Authors: Moore, M. A. S.; Hoskins, I.
Article Title: Ex vivo expansion of cord blood-derived stem cells and progenitors
Abstract: Cord blood-derived CD34+ cells have proved superior to adult marrow or elicited peripheral blood in ex vivo cell and progenitor expansion. In addition, cord blood-derived cells with a phenotype and function of early stem cells (e.g., long-term culture-initiating cells) can be expanded in vitro in the presence of a combination of cytokines by 15- to 20-fold over 7-14 days. These observations suggest that ex vivo expansion of cord blood CD34+ cells will permit improved engraftment of adults and will prove effective in retroviral-mediated gene transfection of early stem cell populations.
Keywords: adult; controlled study; human cell; conference paper; cell proliferation; cells, cultured; cell cycle; cell division; cd34 antigen; stem cell factor; stem cell transplantation; gene expression regulation; cytokine; cytokines; fetal blood; recombinant proteins; newborn; hematopoietic stem cells; umbilical cord blood; recombinant granulocyte colony stimulating factor; autograft; colony-forming units assay; cell separation; granulocyte colony-stimulating factor; recombinant erythropoietin; recombinant granulocyte macrophage colony stimulating factor; interleukins; recombinant interleukin 3; autologous transplantation; granulocyte-macrophage colony-stimulating factor; ex vivo expansion; human; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; recombinant interleukin 6; hematopoietic cell growth factors; cell surface antigens; recombinant interleukin 1beta
Journal Title: Blood Cells
Volume: 20
Issue: 2-3
ISSN: 0340-4684
Publisher: Springer Verlag  
Date Published: 1994-01-01
Start Page: 468
End Page: 479; discussion 479-481
Language: English
PUBMED: 7538352
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028584227&partnerID=40&md5=cc02a51caca0dab22c64e2f7a771069f
Notes: Source: Scopus
Citation Impact
What is Dimensions Citation Badge?
MSK Authors
  1. Malcolm A S Moore
    549 Moore
Related MSK Work