Heterogeneity and clinical significance of ETV1 translocations in human prostate cancer Journal Article
| Authors: | Attard, G.; Clark, J.; Ambroisine, L.; Mills, I. G.; Fisher, G.; Flohr, P.; Reid, A.; Edwards, S.; Kovacs, G.; Berney, D.; Foster, C.; Massie, C. E.; Fletcher, A.; de Bono, J. S.; Scardino, P.; Cuzick, J.; Cooper, C. S. |
| Article Title: | Heterogeneity and clinical significance of ETV1 translocations in human prostate cancer |
| Abstract: | A fluorescence in situ hybridisation (FISH) assay has been used to screen for ETV1 gene rearrangements in a cohort of 429 prostate cancers from patients who had been diagnosed by trans-urethral resection of the prostate. The presence of ETV1 gene alterations (found in 23 cases, 5.4%) was correlated with higher Gleason Score (P=0.001), PSA level at diagnosis (P=<0.0001) and clinical stage (P=0.017) but was not linked to poorer survival. We found that the six previously characterised translocation partners of ETV1 only accounted for 34% of ETV1 re-arrangements (eight out of 23) in this series, with fusion to the androgen-repressed gene C15orf21 representing the commonest event (four out of 23). In 5′-RACE experiments on RNA extracted from formalin-fixed tissue we identified the androgen-upregulated gene ACSL3 as a new 5′-translocation partner of ETV1. These studies report a novel fusion partner for ETV1 and highlight the considerable heterogeneity of ETV1 gene rearrangements in human prostate cancer. © 2008 Cancer Research. |
| Keywords: | adult; controlled study; human tissue; aged; dna binding protein; gene translocation; major clinical study; genetics; dna-binding proteins; androgen; prostate specific antigen; gene; in situ hybridization, fluorescence; reverse transcription polymerase chain reaction; cohort studies; cohort analysis; transcription factor; pathology; transcription factors; prostate cancer; gleason score; prostatic neoplasms; rna; fluorescence in situ hybridization; statistical significance; gene rearrangement; reverse transcriptase polymerase chain reaction; prostate tumor; gene fusion; translocation, genetic; gene control; dna flanking region; formaldehyde; rna extraction; genetic heterogeneity; paraffin; paraffin embedding; open reading frame; transcription factor er81; acsl3; acsl3:etv1 fusion; etv1; etv1 protein, human; long chain fatty acid coenzyme a ligase; long-chain-fatty-acid-coa ligase; acsl3 gene; coenzyme a ligases |
| Journal Title: | British Journal of Cancer |
| Volume: | 99 |
| Issue: | 2 |
| ISSN: | 0007-0920 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2008-07-22 |
| Start Page: | 314 |
| End Page: | 320 |
| Language: | English |
| DOI: | 10.1038/sj.bjc.6604472 |
| PUBMED: | 18594527 |
| PROVIDER: | scopus |
| PMCID: | PMC2480965 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 32" - "Export Date: 17 November 2011" - "CODEN: BJCAA" - "Source: Scopus" |
