Epigenetic lesions at the H19 locus in Wilms' tumour patients Journal Article
| Authors: | Moulton, T.; Crenshaw, T.; Hao, Y.; Moosikasuwan, J.; Lin, N.; Dembitzer, F.; Hensle, T.; Weiss, L.; McMorrow, L.; Loew, T.; Kraus, W.; Gerald, W.; Tycko, B. |
| Article Title: | Epigenetic lesions at the H19 locus in Wilms' tumour patients |
| Abstract: | To test the potential role of H19 as a tumour suppressor gene we have examined its expression and DNA methylation in Wilms' tumours (WTs). In most WTs (18/25), H19 RNA was reduced at least 20–fold from fetal kidney levels. Of the expression–negative tumours ten retained 11p15.5 heterozygosity: in nine of these, H19 DNA was biallelically hypermethylated and in two cases hypermethylation locally restricted to H19 sequences was also present in the non–neoplastic kidney parenchyma. IGF2 mRNA was expressed in most but not all WTs and expression patterns were consistent with IGF2/H19 enhancer competition without obligate inverse coupling. These observations implicate genetic and epigenetic inactivation of H19 in Wilms' tumorigenesis. © 1994 Nature Publishing Group. |
| Keywords: | controlled study; human tissue; methylation; genes; gene expression; genotype; alleles; transcription, genetic; dna methylation; kidney neoplasms; oncogenes; homozygosity; tumor suppressor gene; gene expression regulation, neoplastic; kidney; dna; messenger rna; rna, messenger; dna, neoplasm; genes, ras; rna, neoplasm; genomic imprinting; dna determination; repetitive sequences, nucleic acid; genes, tumor suppressor; nephroblastoma; rna analysis; insulin-like growth factor ii; wilms tumor; enhancer elements (genetics); humans; human; male; female; priority journal; article |
| Journal Title: | Nature Genetics |
| Volume: | 7 |
| Issue: | 3 |
| ISSN: | 1061-4036 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1994-07-01 |
| Start Page: | 440 |
| End Page: | 447 |
| Language: | English |
| DOI: | 10.1038/ng0794-440 |
| PROVIDER: | scopus |
| PUBMED: | 7920666 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
