Effect of epidermal growth factor on the growth and radiation sensitivity of human breast cancer cells in vitro Journal Article
| Authors: | Wollman, R.; Yahalom, J.; Maxy, R.; Pinto, J.; Fuks, Z. |
| Article Title: | Effect of epidermal growth factor on the growth and radiation sensitivity of human breast cancer cells in vitro |
| Abstract: | Purpose: To study the effect of epidermal growth factor (EGF) on the radiation sensitivity of MCF-7 breast cancer cells. Methods and Materials: Radiation dose survival curves were generated for MCF-7 cells under conditions of hormonal deprivation. Epidermal growth factor and/or a monoclonal antibody to its receptor (mAb-225) were added prior to irradiation. Cell cycle distribution was determined by flow cytometry and cellular glutathione (GSH) levels were measured by a glutathione reductase assay. Results: Under hormonal deprivation (control), more than 90% of the MCF-7 cells were arrested in Go/G1 phase and the Do of their survival curve was 0.66 ± .01 Gy. The addition of EGF resulted in (a) growth stimulation; (b) increased percentage of cells in the S-phase of the cell cycle; (c) increased radioresistance (Do = 0.81 ± .04 Gy; p < .05, compared with controls); (d) increased cellular GSH level. The EGF effect on radiation response was observed in a time- and dose-dependent manner. The addition of mAb-225 blocked the ability of EGF to enhance growth and radioresistance (Do = 0.68 ± .03 Gy). Conclusion: Epidermal growth factor stimulates the growth and when administered prior to irradiation increases the radioresistance of hormone-deprived MCF-7 cells. These effects are inhibited by a specific antibody to the EGF receptor. Epidermal growth factor concomitantly increased the fraction of S-phase cells and intracellular GSH levels. This system of growth factor-altered radiosensitivity in human breast cancer cells provides a useful model for the study of the radiation response mechanisms in human malignancy. © 1994. |
| Keywords: | epidermal growth factor; controlled study; human cell; cancer growth; comparative study; radiation dose; flow cytometry; cell survival; cell cycle; cell division; breast cancer; radiation; culture medium; receptor, epidermal growth factor; cancer cell culture; tumor cells, cultured; breast neoplasms; radiation response; dose-response relationship, radiation; monoclonal antibody; antibodies, monoclonal; radiosensitivity; cell cycle phase; cell strain mcf 7; glutathione; culture media; hormones; neoplasms, hormone-dependent; radiation tolerance; dose time effect relation; growth stimulation; human; priority journal; article; glutathione reductase; mcf-7; recombinant epidermal growth factor |
| Journal Title: | International Journal of Radiation Oncology, Biology, Physics |
| Volume: | 30 |
| Issue: | 1 |
| ISSN: | 0360-3016 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1994-08-30 |
| Start Page: | 91 |
| End Page: | 98 |
| Language: | English |
| DOI: | 10.1016/0360-3016(94)90523-1 |
| PROVIDER: | scopus |
| PUBMED: | 8083133 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
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