Molecular cloning of the B-CAM cell surface glycoprotein of epithelial cancers: A novel member of the immunoglobulin superfamily Journal Article
| Authors: | Campbell, I. G.; Foulkes, W. D.; Senger, G.; Trowsdale, J.; Garin-Chesa, P.; Rettig, W. J. |
| Article Title: | Molecular cloning of the B-CAM cell surface glycoprotein of epithelial cancers: A novel member of the immunoglobulin superfamily |
| Abstract: | The human F8/G253 antigen, B-CAM, is a cell surface glycoprotein that is expressed with restricted distribution pattern in normal fetal and adult tissuess and is up-regulated following malignant transformation in some cell types. We have isolated a complementary DNA for B-CAM using an expression cloning technique. The complementary DNA (EMBL accession number X80026) encodes a 588-amino acid protein which is a novel member of the immunoglobulin superfamily with a characteristic V-V-C2-C2-C2 immunoglobulin domain structure. This structure has been described previously for the human MUC18 melanoma antigen (31% amino add identity) and chicken and rat versions of a neural adhesion molecule referred to as SC1/DM-GRASP/BEN or KG-CAM, respectively (26% amino add identity). This homology is suggestive of a role for B-CAM in cell-cell or cell-matrix adhesion. The gene for B-CAM has been mapped by fluorescence in situ hybridization to chromosome 19q13.2-133. © 1994, American Association for Cancer Research. All rights reserved. |
| Keywords: | controlled study; nonhuman; animal cell; mouse; in situ hybridization, fluorescence; neoplasm proteins; nerve tissue proteins; colonic neoplasms; immunoglobulin; tumor cells, cultured; in situ hybridization; cloning, molecular; amino acid sequence; molecular sequence data; membrane glycoproteins; base sequence; chromosomes, human, pair 19; cell adhesion molecules; antigens, cd; sequence homology; protein family; malignant transformation; cell interaction; cell adhesion; blotting, northern; protein determination; carcinoma cell; gene location; complementary dna; cell surface protein; dna, complementary; cell adhesion molecule; neural cell adhesion molecules; chromosome 19q; extracellular matrix proteins; antigens, cd146; humans; priority journal; article; activated-leukocyte cell adhesion molecule |
| Journal Title: | Cancer Research |
| Volume: | 54 |
| Issue: | 22 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1994-11-15 |
| Start Page: | 5761 |
| End Page: | 5765 |
| Language: | English |
| PROVIDER: | scopus |
| PUBMED: | 7954395 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
