Differential effect of pre-transplant cytoreduction on recovery of day zero host circulating cells Journal Article
| Authors: | Papadakis, V.; Mackinnon, S.; Kernan, N. A. |
| Article Title: | Differential effect of pre-transplant cytoreduction on recovery of day zero host circulating cells |
| Abstract: | Host lymphocytes, reactive against donor specific HLA antigens, emerge at the time of graft rejection following T cell-depleted BMT. Thirty patients receiving different cytoreduction regimens were evaluated for the presence of circulating residual, in vitro expandable, host cells on day 0. Twenty-five patients received TBI-containing regimens and 5 patients received non-TBI containing regimens. Patients prepared with thiotepa or TBI-containing regimens had 10-fold lower numbers of circulating host PBMC pre-transplant on day 0 compared with patients cytoreduced with BU/CY. Cell proliferation was observed only in patients who received BU/CY. The phenotype of the in vitro expanded cells was predominately CD3+ (88-97%), CD8+ (45-70%), HLA-DR+ (43-88%), although natural killer cell function and phenotype (CD56+: 6-21%) was also documented. Host cells expanded from a patient who received BU/CY exhibited specific cytotoxicity against feeder cell targets, indicating that BU/CY cytoreduction might not be an optimal BMT preparative regimen for patients at high risk of graft failure. The published experience of similar studies to date is also summarized. |
| Keywords: | clinical article; controlled study; human cell; busulfan; cd3 antigen; cd8 antigen; cell proliferation; t-lymphocytes; phenotype; cells, cultured; carboplatin; etoposide; cyclophosphamide; melphalan; cytotoxicity; thiotepa; risk; graft failure; whole body radiation; blood disease; mononuclear cell; hla dr antigen; natural killer cell; cytotoxicity, immunologic; immunophenotyping; graft rejection; bone marrow transplantation; lymphocyte; host cell; hla antigen; lymphocyte depletion; thymocyte antibody; whole-body irradiation; circulation; diaziquone; cd56 antigen; methylprednisolone sodium succinate; human; male; female; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Bone Marrow Transplantation |
| Volume: | 14 |
| Issue: | 4 |
| ISSN: | 0268-3369 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1994-10-01 |
| Start Page: | 623 |
| End Page: | 630 |
| Language: | English |
| PROVIDER: | scopus |
| PUBMED: | 7858538 |
| DOI/URL: | |
| Notes: | Source: Scopus |
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