Reverse transcriptase polymerase chain reaction for the Ki-1 anaplastic large cell lymphoma-associated t(2;5) translocation in Hodgkin's disease Journal Article
| Authors: | Ladanyi, M.; Cavalchire, G.; Morris, S. W.; Downing, J.; Filippa, D. A. |
| Article Title: | Reverse transcriptase polymerase chain reaction for the Ki-1 anaplastic large cell lymphoma-associated t(2;5) translocation in Hodgkin's disease |
| Abstract: | Hodgkin's disease (HD) and Ki-1 positive anaplastic large cell lymphoma (Ki-1 ALCL) appear pathologically and immunohistochemically related, and a common histogenesis has been postulated in at least some cases. The breakpoints of the t(2;5) (p23;q35) (p235) translocation, which is reported in about 40% of Ki-1 ALCL, have recently been cloned. They involve a novel tyrosine kinase gene, ALK, at 2p23 and the nucleophosmin gene, NPM, at 5q35. Reverse transcriptase polymerase chain reaction (RT-PCR) using NPM and ALK primers consistently detects a fusion product in Ki.1 ALCL cases with the translocation. To determine if this tumor-specific genetic alteration also occurs in HD, we performed NPM-ALK RT-PCR on RNA samples extracted from 40 lymph node biopsies of HD (25 nodular sclerosis, 11 mixed cellularity, 2 lymphocyte depleted, 2 lymphocyte predominant). Using control samples, the sensitivity of the NPM-ALK RT-PCR assay was shown to be at least 1:104. Amplifiable template was confirmed in all samples by RT-PCR using β-actin primers. None of the 40 cases showed the expected 177-bp RT-PCR product indicative of the translocation. We conclude that the most common primary genetic alteration in Ki-1 ALCL, the t(2;5), is absent or very infrequent in typical cases of HD. These results further support the concept that HD and Ki-1 ALCL are pathogenetically distinct entities. |
| Keywords: | controlled study; human tissue; lymph nodes; polymerase chain reaction; protein tyrosine kinase; biopsy; hodgkin disease; rna; molecular sequence data; base sequence; translocation, genetic; large cell lymphoma; anaplastic carcinoma; cd30 antigen; beta actin; nucleophosmin; chromosome 5q; molecular probes; chromosome 2p; lymphoma, large-cell, ki-1; chromosome translocation 2; chromosome translocation 5; rna directed dna polymerase; humans; human; priority journal; article; rna-directed dna polymerase |
| Journal Title: | American Journal of Pathology |
| Volume: | 145 |
| Issue: | 6 |
| ISSN: | 0002-9440 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 1994-12-01 |
| Start Page: | 1296 |
| End Page: | 1300 |
| Language: | English |
| PROVIDER: | scopus |
| PMCID: | PMC1887484 |
| PUBMED: | 7527617 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
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