Circulating cell-free DNA for non-invasive cancer management Journal Article
| Authors: | Stewart, C. M.; Tsui, D. W. Y. |
| Article Title: | Circulating cell-free DNA for non-invasive cancer management |
| Abstract: | Cell-free DNA (cfDNA) was first identified in human plasma in 1948 and is thought to be released from cells throughout the body into the circulatory system. In cancer, a portion of the cfDNA originates from tumour cells, referred to as circulating-tumour DNA (ctDNA), and can contain mutations corresponding to the patient's tumour, for instance specific TP53 alleles. Profiling of cfDNA has recently become an area of increasing clinical relevance in oncology, in particular due to advances in the sensitivity of molecular biology techniques and development of next generation sequencing technologies, as this allows tumour mutations to be identified and tracked non-invasively. This has opened up new possibilities for monitoring tumour evolution and acquisition of resistance, as well as for guiding treatment decisions when tumour biopsy tissue is insufficient or unavailable. In this review, we will discuss the biology of cell-free nucleic acids, methods of analysis, and the potential clinical uses of these techniques, as well as the on-going clinical development of ctDNA assays. © 2018 |
| Keywords: | unclassified drug; review; polymerase chain reaction; sensitivity analysis; allele; metastasis; tumor volume; cancer screening; evolution; cancer resistance; cell heterogeneity; early cancer; early diagnosis; non invasive procedure; dna determination; mutant; sequencing; molecular pathology; exosome; molecular diagnostics; next generation sequencing; cancer; human; priority journal; circulating tumor dna; liquid biopsy; cell-free dna; cell free nucleic acid; cell free rna; circulating cell free dna; prenatal screening |
| Journal Title: | Cancer Genetics |
| Volume: | 228-229 |
| ISSN: | 2210-7762 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2018-12-01 |
| Start Page: | 169 |
| End Page: | 179 |
| Language: | English |
| DOI: | 10.1016/j.cancergen.2018.02.005 |
| PROVIDER: | scopus |
| PUBMED: | 29625863 |
| PMCID: | PMC6598437 |
| DOI/URL: | |
| Notes: | Cancer Genet. -- Cited By :4 -- Export Date: 2 January 2019 -- Review -- Source: Scopus C2 - 29625863 |
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