Recovery and biodistribution of Ex vivo expanded human erythroblasts injected into NOD/SCID/IL2Rγnull mice Journal Article


Authors: Migliaccio, A. R.; Ghinassi, B.; Ferro, L.; Masiello, F.; Tirelli, V.; Sanchez, M.; Migliaccio, G.; Whitsett, C.; Kachala, S.; Riviere, I.; Sadelain, M.
Article Title: Recovery and biodistribution of Ex vivo expanded human erythroblasts injected into NOD/SCID/IL2Rγnull mice
Abstract: Ex vivo expanded erythroblasts (EBs) may serve as advanced transfusion products provided that lodgment occurs in the macrophage-niche of the marrow permitting maturation. EBs expanded from adult and cord blood expressed the receptors (CXCR4, VLA-4, and P-selectin ligand 1) necessary for interaction with macrophages. However, 4-days following transfusion to intact NOD/SCID/IL2R null mice, CD 235 a p o s EBs were observed inside CD 235 a n e g splenic cells suggesting that they underwent phagocytosis. When splenectomized and intact NOD/SCID/IL2R null mice were transfused using retrovirally labeled human EBs, human cells were visualized by bioluminescence imaging only in splenectomized animals. Four days after injection, human CD 235 a p o s cells were detected in marrow and liver of splenectomized mice but only in spleen of controls. Human CD 235 a p o s erythrocytes in blood remained low in all cases. These studies establish splenectomized NOD/SCID/IL2R null mice as a suitable model for tracking and quantification of human EBs in vivo. © 2011 Barbara Ghinassi et al.
Journal Title: Stem Cells International
Volume: 2011
ISSN: 1687-9678
Publisher: Hindawi Publishing Corporation  
Date Published: 2011-08-16
Start Page: 673752
Language: English
DOI: 10.4061/2011/673752
PROVIDER: scopus
PMCID: PMC3161306
PUBMED: 21912558
DOI/URL:
Notes: --- - "Export Date: 2 November 2011" - "Source: Scopus"
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MSK Authors
  1. Stefan S Kachala
    10 Kachala
  2. Michel W J Sadelain
    453 Sadelain
  3. Isabelle C Riviere
    161 Riviere
  4. Leda C Ferro
    4 Ferro