Recovery and biodistribution of Ex vivo expanded human erythroblasts injected into NOD/SCID/IL2Rγnull mice Journal Article
| Authors: | Migliaccio, A. R.; Ghinassi, B.; Ferro, L.; Masiello, F.; Tirelli, V.; Sanchez, M.; Migliaccio, G.; Whitsett, C.; Kachala, S.; Riviere, I.; Sadelain, M. |
| Article Title: | Recovery and biodistribution of Ex vivo expanded human erythroblasts injected into NOD/SCID/IL2Rγnull mice |
| Abstract: | Ex vivo expanded erythroblasts (EBs) may serve as advanced transfusion products provided that lodgment occurs in the macrophage-niche of the marrow permitting maturation. EBs expanded from adult and cord blood expressed the receptors (CXCR4, VLA-4, and P-selectin ligand 1) necessary for interaction with macrophages. However, 4-days following transfusion to intact NOD/SCID/IL2R null mice, CD 235 a p o s EBs were observed inside CD 235 a n e g splenic cells suggesting that they underwent phagocytosis. When splenectomized and intact NOD/SCID/IL2R null mice were transfused using retrovirally labeled human EBs, human cells were visualized by bioluminescence imaging only in splenectomized animals. Four days after injection, human CD 235 a p o s cells were detected in marrow and liver of splenectomized mice but only in spleen of controls. Human CD 235 a p o s erythrocytes in blood remained low in all cases. These studies establish splenectomized NOD/SCID/IL2R null mice as a suitable model for tracking and quantification of human EBs in vivo. © 2011 Barbara Ghinassi et al. |
| Journal Title: | Stem Cells International |
| Volume: | 2011 |
| ISSN: | 1687-9678 |
| Publisher: | Hindawi Publishing Corporation |
| Date Published: | 2011-08-16 |
| Start Page: | 673752 |
| Language: | English |
| DOI: | 10.4061/2011/673752 |
| PROVIDER: | scopus |
| PMCID: | PMC3161306 |
| PUBMED: | 21912558 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 2 November 2011" - "Source: Scopus" |
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