Authors: | Jeffrey, P. D.; Russo, A. A.; Polyak, K.; Gibbs, E.; Hurwitz, J.; Massagué, J.; Pavletich, N. P. |
Article Title: | Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex |
Abstract: | The crystal structure of the human cyclinA-cyclin-dependent kinase2 (CDK2)-ATP complex has been determined at 2.3 Angstrom resolution. CyclinA binds to one side of CDK2's catalytic cleft, inducing large conformational changes in its PSTAIRE helix and T-loop. These changes activate the kinase by realigning active site residues and relieving the steric blockade at the entrance of the catalytic cleft. |
Keywords: | phosphorylation; yeast; cyclins; binding; program; crystal-structure; localization; dependent protein-kinase; invitro; catalytic subunit |
Journal Title: | Nature |
Volume: | 376 |
Issue: | 6538 |
ISSN: | 0028-0836 |
Publisher: | Nature Publishing Group |
Date Published: | 1995-07-27 |
Start Page: | 313 |
End Page: | 320 |
Language: | English |
ACCESSION: | WOS:A1995RL44300040 |
DOI: | 10.1038/376313a0 |
PROVIDER: | wos |
PUBMED: | 7630397 |
Notes: | Article -- Source: Wos |